Dezfuli Bahram S, Manera Maurizio, Giari Luisa, DePasquale Joseph A, Bosi Giampaolo
Department of Life Sciences & Biotechnology, University of Ferrara, St. Borsari 46, 44121 Ferrara, Italy.
Faculty of Biosciences, Agro-Alimentary and Environmental Technologies, University of Teramo, St. Crispi 212, I-64100 Teramo, Italy.
Fish Shellfish Immunol. 2015 Nov;47(1):556-64. doi: 10.1016/j.fsi.2015.09.043. Epub 2015 Oct 3.
A sub-population of 34 specimens of chub, Squalius cephalus, was sampled from the River Brenta (Northern Italy) and examined for ecto- and endo-parasites. Pomphorhynchus laevis (Acanthocephala) was the only enteric helminth encountered. Immunofluorescence and ultrastructural studies were conducted on the intestines of chub. Near the site of parasite's attachment, mucous cells, mast cells (MCs), neutrophils and rodlet cells (RCs) were found to co-occur within the intestinal epithelium. The numbers of mucous cells, MCs and neutrophils were significantly higher in infected fish (Mann-Whitney U test, p < 0.05). Dual immunofluorescence staining with the lectin Dolichos Biflorus Agglutinin (DBA) and the macrophage-specific MAC387 monoclonal antibody, with parallel transmission electron microscopy, revealed that epithelial MCs often made intimate contact with the mucous cells. Degranulation of a large number of MCs around the site of the acanthocephalan's attachment and in proximity to mucous cells was also documented. MCs and neutrophils were abundant in the submucosa. Immune cells of the intestinal epithelium have been described at the ultrastructural level and their possible functions and interactions are discussed.
从意大利北部的布伦塔河采集了34个赤梢鱼样本，对其体内外寄生虫进行了检查。平滑带形吻棘头虫（棘头虫纲）是唯一发现的肠道蠕虫。对赤梢鱼的肠道进行了免疫荧光和超微结构研究。在寄生虫附着部位附近，发现黏液细胞、肥大细胞、中性粒细胞和棒状细胞在肠道上皮中共存。感染鱼体内黏液细胞、肥大细胞和中性粒细胞的数量显著更高（曼-惠特尼U检验，p < 0.05）。用双花扁豆凝集素（DBA）和巨噬细胞特异性MAC387单克隆抗体进行双重免疫荧光染色，并结合透射电子显微镜观察，发现上皮肥大细胞常与黏液细胞紧密接触。还记录到在棘头虫附着部位周围和黏液细胞附近大量肥大细胞脱颗粒的现象。黏膜下层肥大细胞和中性粒细胞丰富。在超微结构水平上描述了肠道上皮的免疫细胞，并讨论了它们可能的功能和相互作用。
