Corral Ariadna, Balcerzyk Marcin, Parrado-Gallego Ángel, Fernández-Gómez Isabel, Lamprea David R, Olmo Alberto, Risco Ramón
Departamento de Física Aplicada III, Universidad de Sevilla, Camino de los Descubrimientos s/n, 41092 Sevilla, Spain.
Centro Nacional de Aceleradores (Universidad de Sevilla - CSIC - Junta de Andalucía), Avenida Tomas Alva Edison 7, 41092 Sevilla, Spain.
Cryobiology. 2015 Dec;71(3):419-31. doi: 10.1016/j.cryobiol.2015.09.007. Epub 2015 Oct 21.
Cryoprotection of bulky organs is crucial for their storage and for subsequent transplantation. In this work we demonstrate the capability of the X-ray computed tomography (CT) as a non-invasive method to measure the cryoprotectant (cpa) concentration inside a tissue or an organ, specifically for the case of dymethil sulfoxide (Me2SO). It is remarkable that the use of Me2SO has been leader in techniques of cells and tissues cryopreservation. Although CT technologies are mainly based in density differences, and many cpas are alcohols with densities similar to water, the use of very low energies as acceleration voltage (∼70 kV) and the sulfur atom in the molecule of Me2SO makes possible the visualization of this cpa inside tissues. As result we obtain a CT signal proportional to the Me2SO concentration with a spatial resolution up to 50 μm in the case of our device.
对大型器官进行冷冻保护对于其储存及后续移植至关重要。在这项工作中，我们展示了X射线计算机断层扫描（CT）作为一种非侵入性方法来测量组织或器官内部冷冻保护剂（CPA）浓度的能力，特别是针对二甲基亚砜（Me2SO）的情况。值得注意的是，Me2SO的使用在细胞和组织冷冻保存技术中一直处于领先地位。尽管CT技术主要基于密度差异，且许多CPA是密度与水相似的醇类，但使用非常低的能量作为加速电压（约70 kV）以及Me2SO分子中的硫原子使得在组织内部可视化这种CPA成为可能。结果，在我们的设备中，我们获得了与Me2SO浓度成正比的CT信号，空间分辨率高达50μm。
