De Kok A J, Sips H, Den Engelse L, Simons J W
Department of Radiation Genetics, Sylvius Laboratories, State University of Leiden, The Netherlands.
Carcinogenesis. 1989 Feb;10(2):237-40. doi: 10.1093/carcin/10.2.237.
To investigate the genetic mechanisms involved in morphological transformation of Syrian hamster embryo (SHE) cells, we have studied the transforming potential of 3-aminobenzamide (3-AB). It was found that prolonged exposure to 3-AB induced morphological transformation of SHE cells as well as C3H10T 1/2 cells. At similar doses, 3-AB induced SCEs and chromosomal alterations (gaps, breaks and exchanges) in SHE cells, but no mutations at the hypoxanthine-guanine phosphoribosyl transferase locus. These data strongly suggest morphological transformation can result from genetic alterations other than gene mutations. The possible nature of these genetic alterations involved will be discussed.
为了研究叙利亚仓鼠胚胎（SHE）细胞形态转化所涉及的遗传机制，我们研究了3-氨基苯甲酰胺（3-AB）的转化潜力。发现长时间暴露于3-AB会诱导SHE细胞以及C3H10T 1/2细胞发生形态转化。在相似剂量下，3-AB在SHE细胞中诱导了姐妹染色单体交换（SCE）和染色体改变（裂隙、断裂和交换），但在次黄嘌呤-鸟嘌呤磷酸核糖转移酶位点未诱导出突变。这些数据强烈表明形态转化可能由基因突变以外的遗传改变引起。将讨论所涉及的这些遗传改变的可能性质。
