致病性小肠结肠炎耶尔森菌、假结核耶尔森菌和鼠疫耶尔森菌中OmpA的同源性分析及交叉免疫原性

Homology analysis and cross-immunogenicity of OmpA from pathogenic Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis.

作者信息

Chen Yuhuang, Duan Ran, Li Xu, Li Kewei, Liang Junrong, Liu Chang, Qiu Haiyan, Xiao Yuchun, Jing Huaiqi, Wang Xin

机构信息

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China.

出版信息

Mol Immunol. 2015 Dec;68(2 Pt A):290-9. doi: 10.1016/j.molimm.2015.09.016. Epub 2015 Oct 3.

DOI:10.1016/j.molimm.2015.09.016

PMID:26435220

Abstract

The outer membrane protein A (OmpA) is one of the intra-species conserved proteins with immunogenicity widely found in the family of Enterobacteriaceae. Here we first confirmed OmpA is conserved in the three pathogenic Yersinia: Yersinia pestis, Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica, with high homology at the nucleotide level and at the amino acid sequence level. The identity of ompA sequences for 262 Y. pestis strains, 134 Y. pseudotuberculosis strains and 219 pathogenic Y. enterocolitica strains are 100%, 98.8% and 97.7% similar. The main pattern of OmpA of pathogenic Yersinia are 86.2% and 88.8% identical at the nucleotide and amino acid sequence levels, respectively. Immunological analysis showed the immunogenicity of each OmpA and cross-immunogenicity of OmpA for pathogenic Yersinia where OmpA may be a vaccine candidate for Y. pestis and other pathogenic Yersinia.

摘要

外膜蛋白A（OmpA）是一种种内保守蛋白，具有免疫原性，广泛存在于肠杆菌科中。在此，我们首次证实OmpA在三种致病性耶尔森菌中保守：鼠疫耶尔森菌、假结核耶尔森菌和致病性小肠结肠炎耶尔森菌，在核苷酸水平和氨基酸序列水平上具有高度同源性。262株鼠疫耶尔森菌、134株假结核耶尔森菌和219株致病性小肠结肠炎耶尔森菌的ompA序列同一性分别为100%、98.8%和97.7%。致病性耶尔森菌OmpA的主要模式在核苷酸和氨基酸序列水平上分别有86.2%和88.8%的同一性。免疫分析显示了每种OmpA的免疫原性以及OmpA对致病性耶尔森菌的交叉免疫原性，其中OmpA可能是鼠疫耶尔森菌和其他致病性耶尔森菌的候选疫苗。