采用二辛可宁酸法和邻苯三酚红钼酸法对尿总蛋白进行比较分析。
Comparative Analysis of Urinary Total Proteins by Bicinchoninic Acid and Pyrogallol Red Molybdate Methods.
作者信息
Yalamati Padma, Bhongir Aparna Varma, Karra Madhulatha, Beedu Sashidhar Rao
机构信息
Associate Professor, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India .
Professor/HOD, Department of Biochemistry, Mediciti Institute of Medical Sciences , Hyderabad, India .
出版信息
J Clin Diagn Res. 2015 Aug;9(8):BC01-4. doi: 10.7860/JCDR/2015/13543.6313. Epub 2015 Aug 1.
BACKGROUND
The concentration of total proteins in urine is a good index of renal function, but its determination is found to be unreliable. The pyrogallol red molybdate (PRM) method for urine total proteins is being widely used in most of the hospitals because of its high sensitivity, better precision and its practicability. Bicinchoninic acid method (BCA) is also used for protein estimation and there have been no studies comparing this method with the PRM method in human urine samples. BCA method overestimates the urinary protein concentration in the presence of interfering substances. After removing the interfering substances present in the human urine samples the results of BCA method were compared with the PRM method.
AIM
The purpose of the study is to identify whether the results of urine total proteins by BCA method are comparable to PRM method and can be used as an alternative to the PRM method.
SETTING AND DESIGN
This is a cross-sectional study done on fresh urine specimens from the hospital laboratory, covering a wide range of protein concentrations.
MATERIAL AND METHODS
Fresh urine specimens covering a wide range of protein concentrations (urine dipstick: nil, trace, 1+, 2+ and ≥ 3+) of 36 patients were analysed by both the methods.
STATISTICAL ANALYSIS
Imprecision was determined by repeated analysis study and Inaccuracy was assessed by comparing the results of the patient's urine samples by both the methods using correlation plots, Bland and Altman, and Passing and Bablok regression analyses.
RESULTS
The coefficient of variation and mean (SD) for the BCA method were 4.6% and 799.1 (882.5) mg/L and for the PRM method were 5.1% and 802.1 (911.9) mg/L. The Pearson correlation coefficient, r was 0.93 (p < 0.0001). Method agreement studies showed no significant constant and proportional bias between both the methods.
CONCLUSION
In urine which is subjected to removal of interfering substances, the BCA results are comparable to PRM method.
背景
尿中总蛋白浓度是肾功能的一个良好指标，但发现其测定结果不可靠。由于其高灵敏度、更好的精密度和实用性，焦性没食子酸红钼酸盐（PRM）法在大多数医院被广泛用于检测尿总蛋白。双缩脲法（BCA）也用于蛋白质定量，且尚无在人尿样本中将该方法与PRM法进行比较的研究。在存在干扰物质的情况下，BCA法会高估尿蛋白浓度。去除人尿样本中存在的干扰物质后，将BCA法的结果与PRM法进行比较。
目的
本研究的目的是确定BCA法检测尿总蛋白的结果是否与PRM法可比，是否可作为PRM法的替代方法。
设置与设计
这是一项对医院实验室新鲜尿标本进行的横断面研究，涵盖了广泛的蛋白浓度范围。
材料与方法
用两种方法对36例患者的新鲜尿标本进行分析，这些标本涵盖了广泛的蛋白浓度范围（尿试纸条检测结果：阴性、微量、1+、2+和≥3+）。
统计分析
通过重复分析研究确定不精密度，并使用相关图、布兰德-奥特曼法以及帕辛-巴布洛克回归分析，比较两种方法对患者尿样本的检测结果来评估不准确度。
结果
BCA法的变异系数和均值（标准差）分别为4.6%和799.1（882.5）mg/L，PRM法的变异系数和均值（标准差）分别为5.1%和802.1（911.9）mg/L。皮尔逊相关系数r为0.93（p<0.0001）。方法一致性研究表明，两种方法之间无显著的恒定偏差和比例偏差。
结论
在去除干扰物质的尿液中，BCA法的结果与PRM法可比。
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