三种检测低流行人群中隐孢子虫卵囊方法的比较。
Comparison of three methods for detection of Cryptosporidium oocysts in a low-prevalence population.
作者信息
Baron E J, Schenone C, Tanenbaum B
机构信息
North Shore University Hospital, Manhasset, New York 11030.
出版信息
J Clin Microbiol. 1989 Jan;27(1):223-4. doi: 10.1128/jcm.27.1.223-224.1989.
Abstract
Four hundred fecal specimens which had been received for routine ova and parasite examination were concentrated by Formalin-ether sedimentation. Sediments were examined as saline and iodine-stained wet preparations and were stained with rhodamine-auramine O and a commercially available monoclonal fluorescent-antibody stain for oocysts of Cryptosporidium species. Examination with the fluorescent stains detected cryptosporidia in both positive specimens (0.5% prevalence), and routine direct wet-preparation examination detected cryptosporidia in one of them. Detection of only low numbers of positive specimens in our nonrisk population argues against routine use of specific and expensive stain reagents.
摘要
四百份用于常规虫卵和寄生虫检查的粪便标本采用福尔马林-乙醚沉淀法进行浓缩。沉淀物分别制成生理盐水涂片和碘染色湿片进行检查，并用罗丹明-金胺O及一种市售的隐孢子虫属卵囊单克隆荧光抗体染色剂进行染色。荧光染色检查在两份阳性标本中均检测到了隐孢子虫（患病率为0.5%），而常规直接湿片检查仅在其中一份标本中检测到了隐孢子虫。在我们的非高危人群中仅检测到少量阳性标本，这表明不支持常规使用特异性强且昂贵的染色试剂。
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