Kass L
Am J Clin Pathol. 1977 Jan;67(1):53-6. doi: 10.1093/ajcp/67.1.53.
Utilizing a unique ability of homocysteine to form a yellow-brown precipitate with nickel chloride, a cytochemical test was developed in an effort to identify this amino acid. Among a variety of types of anemias studied, bright yellow-colored erythrocytes and erythroid precursors were found only in marrows from patients who had untreated pernicious anemia and chronic erythremic myelosis. The results of the study support the "methyltetrahydrofolate-trap" hypothesis in vitamin B12 deficiency, in which decreased activity of the methylocobalamin-dependent methyltransferase enzyme is believed to lead to accumulation of methyltetrahydrofolate and homocysteine in the deficient cells. The findings also raise the possibility that similar intracellular accumulations of homocysteine may occur in chronic erythremic myelosis, perhaps as a result of a defect in the methyltransferase enzyme.
利用同型半胱氨酸与氯化镍形成黄棕色沉淀的独特能力，开发了一种细胞化学测试以鉴定这种氨基酸。在所研究的多种贫血类型中，仅在患有未经治疗的恶性贫血和慢性红细胞增多性骨髓化生患者的骨髓中发现亮黄色的红细胞和红系前体细胞。该研究结果支持维生素B12缺乏症中的“甲基四氢叶酸陷阱”假说，其中钴胺素依赖性甲基转移酶活性降低被认为会导致甲基四氢叶酸和同型半胱氨酸在缺乏细胞中积累。这些发现还增加了慢性红细胞增多性骨髓化生中可能发生类似的同型半胱氨酸细胞内积累的可能性，这可能是由于甲基转移酶存在缺陷所致。
