Johnson Sarah A, Javurek Angela B, Painter Michele S, Ellersieck Mark R, Welsh Thomas H, Camacho Luísa, Lewis Sherry M, Vanlandingham Michelle M, Ferguson Sherry A, Rosenfeld Cheryl S
Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, United States; Biomedical Sciences, University of Missouri, Columbia, MO 65211, United States.
Agriculture Experimental Station-Statistics, University of Missouri, Columbia, MO 65211, United States.
Horm Behav. 2016 Apr;80:139-148. doi: 10.1016/j.yhbeh.2015.09.005. Epub 2015 Oct 5.
Bisphenol A (BPA) is a ubiquitous industrial chemical used in the production of a wide variety of items. Previous studies suggest BPA exposure may result in neuro-disruptive effects; however, data are inconsistent across animal and human studies. As part of the Consortium Linking Academic and Regulatory Insights on BPA Toxicity (CLARITY-BPA), we sought to determine whether female and male rats developmentally exposed to BPA demonstrated later spatial navigational learning and memory deficits. Pregnant NCTR Sprague-Dawley rats were orally dosed from gestational day 6 to parturition, and offspring were directly orally dosed until weaning (postnatal day 21). Treatment groups included a vehicle control, three BPA doses (2.5μg/kg body weight (bw)/day-[2.5], 25μg/kg bw/day-[25], and 2500μg/kg bw/day-[2500]) and a 0.5μg/kg/day ethinyl estradiol (EE)-reference estrogen dose. At adulthood, 1/sex/litter was tested for seven days in the Barnes maze. The 2500 BPA group sniffed more incorrect holes on day 7 than those in the control, 2.5 BPA, and EE groups. The 2500 BPA females were less likely than control females to locate the escape box in the allotted time (p value=0.04). Although 2.5 BPA females exhibited a prolonged latency, the effect did not reach significance (p value=0.06), whereas 2.5 BPA males showed improved latency compared to control males (p value=0.04), although the significance of this result is uncertain. No differences in serum testosterone concentration were detected in any male or female treatment groups. Current findings suggest developmental exposure of rats to BPA may disrupt aspects of spatial navigational learning and memory.
双酚A（BPA）是一种广泛存在的工业化学品，用于生产各种各样的物品。先前的研究表明，接触双酚A可能会导致神经干扰效应；然而，动物和人类研究的数据并不一致。作为双酚A毒性的学术与监管见解联合研究项目（CLARITY - BPA）的一部分，我们试图确定在发育过程中接触双酚A的雌性和雄性大鼠是否会出现后期空间导航学习和记忆缺陷。怀孕的NCTR斯普拉格 - 道利大鼠从妊娠第6天到分娩期间经口给药，后代直接经口给药直至断奶（出生后第21天）。治疗组包括溶剂对照组、三个双酚A剂量组（2.5微克/千克体重（bw）/天 - [2.5]、25微克/千克bw/天 - [25]和2500微克/千克bw/天 - [2500]）以及一个0.5微克/千克/天乙炔雌二醇（EE） - 参考雌激素剂量组。成年后，每个性别/窝选取1只大鼠在巴恩斯迷宫中进行为期7天的测试。2500双酚A组在第7天嗅闻的错误洞数比对照组、2.5双酚A组和EE组更多。2500双酚A组的雌性大鼠在规定时间内找到逃生箱的可能性低于对照组雌性大鼠（p值 = 0.04）。虽然2.5双酚A组的雌性大鼠潜伏期延长，但该效应未达到显著水平（p值 = 0.06），而2.5双酚A组的雄性大鼠与对照组雄性大鼠相比潜伏期有所改善（p值 = （0.04），尽管该结果的显著性尚不确定。在任何雄性或雌性治疗组中均未检测到血清睾酮浓度的差异。目前的研究结果表明，大鼠在发育过程中接触双酚A可能会扰乱空间导航学习和记忆的某些方面。
