Routila Johannes, Bilgen Türker, Saramäki Outi, Grénman Reidar, Visakorpi Tapio, Westermarck Jukka, Ventelä Sami
Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
Prostate Cancer Research Center, Institute of Biosciences and Medical Technology-BioMediTech, University of Tampere, Tampere, Finland.
J Oral Pathol Med. 2016 May;45(5):329-37. doi: 10.1111/jop.12372. Epub 2015 Oct 5.
CIP2A, an inhibitor of PP2A tumour suppressor function, is a widely overexpressed biomarker of aggressive disease and poor therapy response in multiple human cancer types.
CIP2A and DPPA4 copy number alterations and expression were analysed by fluorescence in situ hybridisation (FISH) and immunohistochemistry (IHC) in different cell lines and a tissue microarray of 52 HNSCC patients. Results were correlated with patient survival and other clinicopathological data.
CIP2A and DPPA4 copy number increase occurred at a relatively high frequency in human HNSCC patient samples. CIP2A but not DPPA4 FISH status was significantly associated with patient survival. CIP2A detection by combining IHC with FISH yielded superior resolution in the prognostication of HNSCC.
CIP2A copy number increase is associated with poor patient survival in human HNSCC. We suggest that the reliability and prognostic value of CIP2A detection can be improved by performing FISH analysis to CIP2A IHC positive tumours.
CIP2A是PP2A肿瘤抑制功能的抑制剂，在多种人类癌症类型中是侵袭性疾病和治疗反应不佳的广泛过表达生物标志物。
通过荧光原位杂交（FISH）和免疫组织化学（IHC）分析不同细胞系和52例头颈部鳞状细胞癌（HNSCC）患者组织芯片中CIP2A和DPPA4的拷贝数改变及表达情况。结果与患者生存率及其他临床病理数据相关。
在人类HNSCC患者样本中，CIP2A和DPPA4拷贝数增加的发生率相对较高。CIP2A而非DPPA4的FISH状态与患者生存率显著相关。联合IHC与FISH检测CIP2A在HNSCC预后评估中具有更高的分辨率。
CIP2A拷贝数增加与人类HNSCC患者的不良生存相关。我们建议，通过对CIP2A IHC阳性肿瘤进行FISH分析，可以提高CIP2A检测的可靠性和预后价值。
