基于癌细胞系芯片的头颈部鳞状细胞癌放射抵抗相关生物标志物筛选方法的研究
Cancer cell line microarray as a novel screening method for identification of radioresistance biomarkers in head and neck squamous cell carcinoma.
机构信息
Turku Bioscience Centre, University of Turku and Åbo Akademi university, Turku, Finland.
Department for Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital and University of Turku, Kiinamyllynkatu 4-8, 20521, Turku, Finland.
出版信息
BMC Cancer. 2021 Jul 29;21(1):868. doi: 10.1186/s12885-021-08618-6.
BACKGROUND
Currently, no clinically useful biomarkers for radioresistance are available in head and neck squamous cell carcinoma (HNSCC). This study assesses the usefulness of Cell Line Microarray (CMA) method to enhance immunohistochemical screening of potential immunohistochemical biomarkers for radioresistance in HNSCC cell lines.
METHODS
Twenty-nine HNSCC cell lines were cultured, cell pellets formalin-fixed, paraffin-embedded, and arrayed. Radioresistance features of the cell lines were combined to immunohistochemical stains for p53, NDFIP1, EGFR, stem cell marker Oct4, and PP2A inhibitor CIP2A.
RESULTS
Expression of p53, EGFR or CIP2A did not indicate intrinsic radioresistance in vitro. Stem cell marker Oct4 nuclear positivity and NDFIP1 nuclear positivity was correlated with increased intrinsic radioresistance.
CONCLUSION
The usefulness of CMA in analysis of HNSCC cell lines and discovery of biomarkers is demonstrated. CMA is very well adapted to both testing of antibodies in a large panel of cell lines as well as correlating staining results with other cell line characteristics. In addition, CMA-based antibody screening proved an efficient and relatively simple method to identify potential radioresistance biomarkers in HNSCC.
背景
目前，头颈部鳞状细胞癌（HNSCC）中没有用于放射抵抗的临床有用的生物标志物。本研究评估了细胞系微阵列（CMA）方法在增强 HNSCC 细胞系中潜在放射抵抗的免疫组织化学生物标志物的免疫组织化学筛选中的作用。
方法
培养了 29 种 HNSCC 细胞系，福尔马林固定，石蜡包埋，并进行了排列。将细胞系的放射抵抗特征与 p53、NDFIP1、EGFR、干细胞标志物 Oct4 和 PP2A 抑制剂 CIP2A 的免疫组织化学染色相结合。
结果
p53、EGFR 或 CIP2A 的表达并不能表明体外固有放射抵抗性。干细胞标志物 Oct4 核阳性和 NDFIP1 核阳性与固有放射抵抗性增加相关。
结论
CMA 用于分析 HNSCC 细胞系和发现生物标志物的用途得到了证明。CMA 非常适合在大量细胞系中测试抗体，以及将染色结果与其他细胞系特征相关联。此外，基于 CMA 的抗体筛选被证明是一种有效的、相对简单的方法，可以鉴定 HNSCC 中的潜在放射抵抗生物标志物。
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