Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
Trends Cell Biol. 2016 Jan;26(1):65-75. doi: 10.1016/j.tcb.2015.08.006. Epub 2015 Oct 1.
The generation of induced pluripotent stem cells (iPSCs) and directly converted cells holds great promise in regenerative medicine. However, after in-depth studies of the murine system, we know that the current methodologies to produce these cells are not ideal and mostly yield cells of poor quality that might hold a risk in therapeutic applications. In this review we address the duality found in the literature regarding the use of 'quality' as a criterion for the clinic. We discuss the elements that influence reprogramming quality, and provide evidence that safety and functionality are directly linked to cell quality. Finally, because most of the available data come from murine systems, we speculate about what aspects can be applied to human cells.
诱导多能干细胞（iPSCs）和直接转化细胞的产生在再生医学中具有巨大的前景。然而，在对鼠系统进行深入研究后，我们知道目前产生这些细胞的方法并不理想，大多数方法产生的细胞质量较差，在治疗应用中可能存在风险。在这篇综述中，我们讨论了文献中关于将“质量”用作临床标准的双重性。我们讨论了影响重编程质量的因素，并提供了证据表明安全性和功能性与细胞质量直接相关。最后，由于大多数可用数据来自鼠系统，我们推测哪些方面可以应用于人类细胞。
