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糖苷水解酶家族32存在于枯草芽孢杆菌噬菌体中。

Glycoside hydrolase family 32 is present in Bacillus subtilis phages.

作者信息

Maaroufi Halim, Levesque Roger C

机构信息

Institut de biologie intégrative et des systèmes (IBIS), Plate-Forme de Bio-Informatique, Université Laval, Pavillon Charles-Eugène Marchand, 1030 Avenue de la médecine, Québec, Québec, G1V 0A6, Canada.

Institut de Biologie Intégrative et des Systèmes (IBIS) and Département de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Québec, G1V 0A6, Canada.

出版信息

Virol J. 2015 Oct 6;12:157. doi: 10.1186/s12985-015-0373-6.

DOI:10.1186/s12985-015-0373-6
PMID:26438422
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4595243/
Abstract

BACKGROUND

Glycoside hydrolase family 32 (GH32) enzymes cleave the glycosidic bond between two monosaccharides or between a carbohydrate and an aglycone moiety. GH32 enzymes have been studied in prokaryotes and in eukaryotes but not in viruses.

FINDINGS

This is the first analysis of GH32 enzymes in Bacillus subtilis phage SP10, ϕNIT1 and SPG24. Phylogenetic analysis, molecular docking and secretability predictions suggest that phage GH32 enzymes function as levan (fructose homopolysaccharide) fructotransferase.

CONCLUSIONS

We showed that viruses also contain GH32 enzymes and that our analyses in silico strongly suggest that these enzymes function as levan fructotransferase.

摘要

背景

糖苷水解酶家族32（GH32）酶可裂解两个单糖之间或碳水化合物与糖苷配基部分之间的糖苷键。已在原核生物和真核生物中对GH32酶进行了研究，但尚未在病毒中进行研究。

研究结果

这是首次对枯草芽孢杆菌噬菌体SP10、ϕNIT1和SPG24中的GH32酶进行分析。系统发育分析、分子对接和分泌性预测表明，噬菌体GH32酶发挥果聚糖（果糖同多糖）果糖转移酶的作用。

结论

我们发现病毒中也含有GH32酶，并且我们的计算机模拟分析有力地表明这些酶发挥果聚糖果糖转移酶的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c6b/4595243/0de80f35f4de/12985_2015_373_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c6b/4595243/1cbba8f954bb/12985_2015_373_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c6b/4595243/49fc77367336/12985_2015_373_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c6b/4595243/0de80f35f4de/12985_2015_373_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c6b/4595243/1cbba8f954bb/12985_2015_373_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c6b/4595243/49fc77367336/12985_2015_373_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c6b/4595243/0de80f35f4de/12985_2015_373_Fig3_HTML.jpg

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