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一种新型枯草芽孢杆菌感染噬菌体BSP38的全核苷酸序列分析,该噬菌体可能属于Spounavirinae亚科中的一个新属。

Complete nucleotide sequence analysis of a novel Bacillus subtilis-infecting phage, BSP38, possibly belonging to a new genus in the subfamily Spounavirinae.

作者信息

Ghosh Kuntal, Kim Kwang-Pyo

机构信息

Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Korea.

出版信息

Arch Virol. 2019 Mar;164(3):875-878. doi: 10.1007/s00705-018-4110-5. Epub 2018 Dec 1.

Abstract

Bacillus subtilis-infecting phage BSP38 was isolated from a sewage sample. Morphologically, BSP38 was found to be similar to members of the subfamily Spounavirinae, family Myoviridae. Its genome is 153,268 bp long with 41.8% G+C content and 254 putative open reading frames (ORFs) as well as six tRNAs. A distinguishing feature for this phage among the reported B. subtilis-infecting phages is the presence of an encoding ORF, putative tRNA guanylyltransferase-like protein. Genomic comparisons with the other reported phages strongly suggest that BSP38 should be considered a member of a new genus in the subfamily Spounavirinae.

摘要

从污水样本中分离出了感染枯草芽孢杆菌的噬菌体BSP38。在形态学上,发现BSP38与肌尾噬菌体科Spounavirinae亚科的成员相似。其基因组长度为153,268 bp,G+C含量为41.8%,有254个推定的开放阅读框(ORF)以及6个tRNA。在已报道的感染枯草芽孢杆菌的噬菌体中,该噬菌体的一个显著特征是存在一个编码推定的tRNA鸟苷酰转移酶样蛋白的ORF。与其他已报道噬菌体的基因组比较强烈表明,BSP38应被视为Spounavirinae亚科一个新属的成员。

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