Oyabu Takako, Morimoto Yasuo, Izumi Hiroto, Yoshiura Yukiko, Tomonaga Taisuke, Lee Byeong-Woo, Okada Takami, Myojo Toshihiko, Shimada Manabu, Kubo Masaru, Yamamoto Kazuhiro, Kawaguchi Kenji, Sasaki Takeshi
University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
Department of Environmental Health Engineering, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi, Kitakyushu, 807-8555, Japan.
Environ Health Prev Med. 2016 Jan;21(1):42-8. doi: 10.1007/s12199-015-0493-z. Epub 2015 Oct 5.
We performed the two inhalation exposures, whole-body inhalation and nose-only inhalation, to investigate the pulmonary deposition and health effects of the two inhalation methods.
In both methods, we exposed rats to the same TiO2 nanoparticles at almost the same exposure concentration for 6 h and compared the deposited amounts of nanoparticles and histopathological changes in the lungs. Rats were exposed to rutile-type TiO2 nanoparticles generated by the spray-dry method for 6 h. The exposure concentration in the whole-body chamber was 4.10 ± 1.07 mg/m(3), and that in nose-only chamber was 4.01 ± 1.11 mg/m(3). The particle sizes were 230 and 180 nm, respectively. A control group was exposed to fresh air.
The amounts of TiO2 deposited in the lungs as measured by ICP-AES after acid digestion just after the exposure were: 42.6 ± 3.5 μg in the whole-body exposure and 46.0 ± 7.7 μg in the nose-only exposure groups. The histopathological evaluation was the same in both exposure groups: no infiltration of inflammatory cells in the alveolar space and interstitium, and no fibrosis.
The two inhalation methods using the same material under the same exposure conditions resulted in the same particle deposition and histopathology in the lung.
我们进行了两种吸入暴露实验，即全身吸入和仅经鼻吸入，以研究这两种吸入方式下的肺部沉积情况及健康影响。
在两种方法中，我们让大鼠在几乎相同的暴露浓度下接触相同的二氧化钛纳米颗粒，持续6小时，并比较纳米颗粒的沉积量以及肺部的组织病理学变化。大鼠暴露于通过喷雾干燥法产生的金红石型二氧化钛纳米颗粒中6小时。全身暴露舱内的暴露浓度为4.10±1.07毫克/立方米，仅经鼻暴露舱内的暴露浓度为4.01±1.11毫克/立方米。颗粒尺寸分别为230纳米和180纳米。对照组暴露于新鲜空气中。
暴露后经酸消解，通过电感耦合等离子体发射光谱法（ICP - AES）测量的肺部二氧化钛沉积量为：全身暴露组为42.6±3.5微克，仅经鼻暴露组为46.0±7.7微克。两个暴露组的组织病理学评估结果相同：肺泡腔和间质中无炎性细胞浸润，无纤维化。
在相同暴露条件下使用相同材料的两种吸入方式，在肺部产生了相同的颗粒沉积和组织病理学结果。
