Wang Z, Gao X Z, Preisler H D
Roswell Park Memorial Institute, Department of Hematologic Oncology, Buffalo, NY 14263.
Am J Hematol. 1989 Feb;30(2):82-5. doi: 10.1002/ajh.2830300206.
Immature myeloid cells prepared from patients with AML were placed into suspension culture and studied over a 2-week period. Cell numbers usually fell and viability was not well maintained. Some degree of differentiation was observed in most cultures. Considering these observations, together with those derived from parallel studies of immature CML cells, the data suggest that AML cells are more dependent than CML cells on environmental conditions for the maintenance of cell viability and proliferation. This is especially the case for AML cells obtained at the time of initial diagnosis. On the other hand, AML cells and myeloid blastic crisis CML cells are similar with respect to their apparent greater ability to differentiate in vitro than in vivo. The addition of recombinant hemopoietins to the suspension cultures of AML cells is associated with either increased proliferation or differentiation but not both. The cells of different patients respond differently to the different hemopoietins and the different hemopoietins produce different affects in cultures of cells obtained from the same patient.
从急性髓系白血病（AML）患者体内制备的未成熟髓细胞被置于悬浮培养中，并在两周时间内进行研究。细胞数量通常会下降，且活力无法得到良好维持。在大多数培养物中观察到了一定程度的分化。综合这些观察结果以及从慢性髓系白血病（CML）未成熟细胞的平行研究中得出的结果，数据表明，AML细胞在维持细胞活力和增殖方面比CML细胞更依赖于环境条件。对于初次诊断时获得的AML细胞而言尤其如此。另一方面，AML细胞和慢性髓系白血病急变期细胞在体外的分化能力明显强于体内这一点上是相似的。向AML细胞的悬浮培养物中添加重组造血因子会导致增殖增加或分化增加，但不会两者同时出现。不同患者的细胞对不同的造血因子反应不同，并且不同的造血因子对从同一患者获得的细胞培养物会产生不同的影响。
