Ademosun Ayokunle O, Oboh Ganiyu, Bello Fatai, Ayeni Peluola O
Federal University of Technology, Akure, Nigeria.
Federal University of Technology, Akure, Nigeria
J Evid Based Complementary Altern Med. 2016 Oct;21(4):NP11-7. doi: 10.1177/2156587215610032. Epub 2015 Oct 4.
This study sought to investigate the anticholinesterase and antioxidative properties of quercetin and its glycosylated conjugate, rutin. The in vitro inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities, inhibition of Fe(2+)-induced lipid peroxidation in rat's brain homogenates, radicals scavenging, and Fe(2+)-chelating abilities of the flavonoids were investigated in vitro with concentrations of the samples ranging from 0.06 to 0.6 mM. Quercetin had significantly higher AChE and BChE inhibitory abilities than rutin. Quercetin also had stronger inhibition of Fe(2+)-induced lipid peroxidation in rat's brain homogenates. Similarly, quercetin had higher radical scavenging abilities than rutin. Quercetin also had stronger Fe(2+)-chelating ability than rutin. The inhibition of cholinesterases and antioxidative properties are possible mechanisms by which the flavonoids can be used in the management of oxidative stress-induced neurodegeneration.
本研究旨在探究槲皮素及其糖基化共轭物芦丁的抗胆碱酯酶和抗氧化特性。在体外研究了黄酮类化合物对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）活性的抑制作用、对大鼠脑匀浆中Fe(2+)诱导的脂质过氧化的抑制作用、自由基清除能力以及Fe(2+)螯合能力，样品浓度范围为0.06至0.6 mM。槲皮素对AChE和BChE的抑制能力明显高于芦丁。槲皮素对大鼠脑匀浆中Fe(2+)诱导的脂质过氧化也有更强的抑制作用。同样，槲皮素的自由基清除能力高于芦丁。槲皮素的Fe(2+)螯合能力也比芦丁更强。胆碱酯酶的抑制作用和抗氧化特性可能是黄酮类化合物用于管理氧化应激诱导的神经退行性变的潜在机制。
