Wuytens Pieter C, Subramanian Ananth Z, De Vos Winnok H, Skirtach Andre G, Baets Roel
Photonics Research Group, INTEC Department, Ghent University-imec, Ghent, Belgium.
Department of Molecular Biotechnology, Ghent University, Ghent, Belgium and Laboratory of Cell Biology and Histology, Dept. Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
Analyst. 2015 Dec 21;140(24):8080-7. doi: 10.1039/c5an01782c.
While top-down substrates for surface-enhanced Raman spectroscopy (SERS) offer outstanding control and reproducibility of the gold nanopatterns and their related localized surface plasmon resonance, intracellular SERS experiments heavily rely on gold nanoparticles. These nanoparticles often result in varying and uncontrollable enhancement factors. Here we demonstrate the use of top-down gold-nanostructured microchips for intracellular sensing. We develop a tunable and reproducible fabrication scheme for these microchips. Furthermore we observe the intracellular uptake of these structures, and find no immediate influence on cell viability. Finally, we perform a proof-of-concept intracellular SERS experiment by the label-free detection of extraneous molecules. By bringing top-down SERS substrates to the intracellular world, we set an important step towards time-dependent and quantitative intracellular SERS.
虽然用于表面增强拉曼光谱（SERS）的自上而下的基底能对金纳米图案及其相关的局域表面等离子体共振实现出色的控制和可重复性，但细胞内SERS实验严重依赖金纳米颗粒。这些纳米颗粒常常导致增强因子变化且不可控。在此，我们展示了将自上而下的金纳米结构微芯片用于细胞内传感。我们为这些微芯片开发了一种可调谐且可重复的制造方案。此外，我们观察到这些结构被细胞摄取，并且发现对细胞活力没有即时影响。最后，我们通过对细胞外分子进行无标记检测开展了一个概念验证性的细胞内SERS实验。通过将自上而下的SERS基底引入细胞内环境，我们朝着实现随时间变化的定量细胞内SERS迈出了重要一步。
