大鼠机械损伤后蛋白质表达（p75NTR、bax、bcl-2和caspase-3）与皮质神经元凋亡的相关性研究

Correlation research on the protein expression (p75NTR, bax, bcl-2, and caspase-3) and cortical neuron apoptosis following mechanical injury in rat.

作者信息

Liu H-C, Zhang Y, Zhang S, Xin T, Li W-H, Wu W-L, Pang Q, Chen Y-Z

机构信息

Department of Orthopedic, Qilu Hospital, Shandong University, Jinan, China.

出版信息

Eur Rev Med Pharmacol Sci. 2015 Sep;19(18):3459-67.

PMID:26439043

Abstract

OBJECTIVE

In this study, we aimed to survey the role of p75NTR, bax, bcl-2, and caspase-3 in the progress of traumatic brain injury (TBI).

MATERIALS AND METHODS

A mechanical trauma model of vital neurons was established by putting external pressure, contusion and centrifugal acceleration on neurons. Morphological change, survival rate, assay of LDH activity, and apoptosis rate were evaluated for mild, medium and severe injury models. The expression of bax, bcl-2, caspase-3, p75NTR, p75NTR mRNA was determined by immunohistochemistry, immunofluorescence, Western blotting and RT-PCR.

RESULTS

There was a transient high level Bcl-2 protein within 2 h after injury to increase neuronal tolerance and avoid apoptosis. Subsequently p75NTR, Bax/Bcl-2, and Caspase-3 reached their peaks from 48 to 72 h accompanied with the maximum apoptosis rate.

CONCLUSIONS

Our results suggest that apoptosis ratio in varying degree injury groups are correlated with the expression level of p75NTRmRNA, p75NTR, Caspase-3, Bax/Bcl-2 ratio.

摘要

目的

在本研究中，我们旨在探究p75神经营养因子受体（p75NTR）、bax、bcl-2和半胱天冬酶-3（caspase-3）在创伤性脑损伤（TBI）进展中的作用。

材料与方法

通过对神经元施加外部压力、挫伤和离心加速度，建立重要神经元的机械创伤模型。对轻度、中度和重度损伤模型评估形态学变化、存活率、乳酸脱氢酶（LDH）活性测定及凋亡率。通过免疫组织化学、免疫荧光、蛋白质印迹法和逆转录-聚合酶链反应（RT-PCR）测定bax、bcl-2、caspase-3、p75NTR、p75NTR mRNA的表达。