Tao Zhi-Peng, Sun Le-Chang, Qiu Xu-Jian, Cai Qiu-Feng, Liu Guang-Ming, Su Wen-Jin, Cao Min-Jie
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, Fujian Province, 361102, China.
J Sci Food Agric. 2016 Jul;96(9):3088-97. doi: 10.1002/jsfa.7484. Epub 2015 Nov 11.
In China, abalone (Haliotis discus hannai) production is growing annually. During industrial processing, the viscera, which are abundant of cellulase, are usually discarded or processed into low-value feedstuff. Thus, it is of interest to obtain cellulase from abalone viscera and investigate its application for preparation of functional oligosaccharides.
A cellulase was purified from the hepatopancreas of abalone by ammonium sulfate precipitation and two-steps column chromatography. The molecular weight of the cellulase was 45 kDa on SDS-PAGE. Peptide mass fingerprinting analysis yielded 103 amino acid residues, which were identical to cellulases from other species of abalone. Substrate specificity analysis indicated that the cellulase is an endo-1,4-β-glucanase. Hydrolysis of seaweed Porphyra haitanensis polysaccharides by the enzyme produced oligosaccharides with degree of polymerisation of two to four, whose monosaccharide composition was 58% galactose, 4% glucose and 38% xylose. The oligosaccharides revealed 2,2'-diphenyl-1-picrylhydrazyl free radical as well as hydrogen peroxide scavenging activity.
It is feasible and meaningful to utilise cellulase from the viscera of abalone for preparation of functional oligosaccharides. © 2015 Society of Chemical Industry.
在中国，皱纹盘鲍的产量逐年增长。在工业加工过程中，富含纤维素酶的内脏通常被丢弃或加工成低价值的饲料。因此，从鲍鱼内脏中获取纤维素酶并研究其在制备功能性低聚糖方面的应用具有重要意义。
通过硫酸铵沉淀和两步柱层析从鲍鱼的肝胰腺中纯化出一种纤维素酶。该纤维素酶在SDS-PAGE上的分子量为45 kDa。肽质量指纹图谱分析得到103个氨基酸残基，与其他种类鲍鱼的纤维素酶相同。底物特异性分析表明该纤维素酶是一种内切-1,4-β-葡聚糖酶。用该酶水解坛紫菜多糖产生聚合度为2至4的低聚糖，其单糖组成为58%的半乳糖、4%的葡萄糖和38%的木糖。这些低聚糖具有清除2,2'-二苯基-1-苦基肼自由基以及过氧化氢的活性。
利用鲍鱼内脏中的纤维素酶制备功能性低聚糖是可行且有意义的。© 2015化学工业协会。
