Marine Biotechnology Research Center, Jeonnam Bioindustry Foundation, 21-7, Nonggongdanji 4Gil, Wando-eup, Wando-gun, Jeollanam-do 59108, Korea.
Medical & Bio Decision (MBD) Co. Ltd., #B-8F, 145 Gwanggyo-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16229, Korea.
Mar Drugs. 2021 Apr 15;19(4):219. doi: 10.3390/md19040219.
Much attention is being devoted to the potential of marine sulfated polysaccharides as antiviral agents in preventing COVID-19. In this study, sulfated fucoidan and crude polysaccharides, extracted from six seaweed species (Undaria pinnatifida sporophyll, Laminaria japonica, Hizikia fusiforme, Sargassum horneri, Codium fragile, Porphyra tenera) and Haliotis discus hannai (abalone viscera), were screened for their inhibitory activity against SARS-CoV-2 virus entry. Most of them showed significant antiviral activities at an IC50 of 12289 μg/mL against SARS-CoV-2 pseudovirus in HEK293/ACE2, except for P. tenera (IC50 > 1000 μg/mL). The crude polysaccharide of S. horneri showed the strongest antiviral activity, with an IC50 of 12 μg/mL, to prevent COVID-19 entry, and abalone viscera and H. fusiforme could also inhibit SARS-CoV-2 infection with an IC50 of 33 μg/mL and 47 μg/mL, respectively. The common properties of these crude polysaccharides, which have strong antiviral activity, are high molecular weight (>800 kDa), high total carbohydrate (62.799.1%), high fucose content (37.3~66.2%), and highly branched polysaccharides. These results indicated that the crude polysaccharides from seaweeds and abalone viscera can effectively inhibit SARS-CoV-2 entry.
人们高度关注海洋硫酸多糖作为预防 COVID-19 的抗病毒药物的潜力。在这项研究中，从六种海藻（裙带菜孢子叶、海带、羊栖菜、美舌藻、脆江蓠、紫菜）和皱纹盘鲍内脏中提取的硫酸岩藻聚糖和粗多糖，被筛选出对 SARS-CoV-2 病毒进入的抑制活性。除了紫菜（IC50＞1000μg/mL）之外，它们中的大多数在 HEK293/ACE2 中对 SARS-CoV-2 假病毒的 IC50 为 12~289μg/mL，显示出显著的抗病毒活性。美舌藻的粗多糖表现出最强的抗病毒活性，IC50 为 12μg/mL，可预防 COVID-19 进入，鲍鱼内脏和羊栖菜也可以分别以 IC50 为 33μg/mL 和 47μg/mL 抑制 SARS-CoV-2 感染。这些具有强抗病毒活性的粗多糖的共同特性是高分子量（＞800kDa）、高总糖（62.7-99.1%）、高岩藻糖含量（37.3-66.2%）和高度支化多糖。这些结果表明，海藻和鲍鱼内脏的粗多糖可以有效抑制 SARS-CoV-2 的进入。
