Zhang Liqin, Zhu Guizhi, Mei Lei, Wu Cuichen, Qiu Liping, Cui Cheng, Liu Yuan, Teng I-Ting, Tan Weihong
Departments of Chemistry, Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida , Gainesville, Florida 32611, United States.
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.
ACS Appl Mater Interfaces. 2015 Nov 4;7(43):24069-74. doi: 10.1021/acsami.5b06987. Epub 2015 Oct 22.
Synthetic unmethylated cytosine-guanine (CpG) oligodeoxynucleotides are immunostimulatory motifs that have shown promise as vaccines or adjuvants for diseases such as cancers and infectious diseases. In the present work, novel immuno-nanoflowers (NFs), self-assembled from long DNA integrated with tandem CpG through rolling circle replication, were developed for efficient CpG delivery and protection from nuclease degradation. In a model of macrophage-like cells, the CpG NFs proved to be potent immunostimulators by triggering the proliferation of these immune cells, which, in turn, secreted immunostimulatory cytokines, including tumor necrosis factor α, interleukin-6, and interleukin-10. These results demonstrate the ability of CpG NFs to induce cancer cell apoptosis and necrosis.
合成的非甲基化胞嘧啶-鸟嘌呤（CpG）寡脱氧核苷酸是免疫刺激基序，已显示出作为癌症和传染病等疾病的疫苗或佐剂的潜力。在本研究中，通过滚环复制从与串联CpG整合的长DNA自组装而成的新型免疫纳米花（NFs）被开发出来，用于高效递送CpG并防止核酸酶降解。在巨噬细胞样细胞模型中，CpG NFs通过触发这些免疫细胞的增殖被证明是有效的免疫刺激剂，这些免疫细胞进而分泌免疫刺激细胞因子，包括肿瘤坏死因子α、白细胞介素-6和白细胞介素-10。这些结果证明了CpG NFs诱导癌细胞凋亡和坏死的能力。
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