Charpin C, Andrac L, Habib M C, Vacheret H, Xerri L, Devictor B, Lavaut M N, Toga M
Department of Pathology, CHU Timone, Marseille, France.
Cancer. 1989 Mar 1;63(5):863-72. doi: 10.1002/1097-0142(19890301)63:5<863::aid-cncr2820630512>3.0.co;2-p.
Immunocytochemical assays (ICA) using monoclonal antiestrogen receptors (ER ICA), antiprogesterone receptors (PR ICA), and monoclonal antibody Ki67 (Ki67 ICA) were performed in 127 breast carcinomas. The immunostaining procedures were applied on frozen tissue sections, tumour imprints, and fine-needle aspirates in order to compare the variations in the distribution of the antigens detected in the three different types of preparations. Positive reactions detected with peroxidase-antiperoxidase and avidinbiotin-peroxidase, and alkaline phosphatase-antialkaline phosphatase complexes were evaluated through a computerized system of image analysis referred to as SAMBA 200 (SAMBA TITN, Grenoble, France). Application programs specifically developed for the analysis of tissue sections and of cytologic preparations were applied. This system allowed a multiparametric, accurate, reliable, reproducible and automatized evaluation of the heterogeneity of the antigenic sites in tumors. For each markers positive cell surface (PS), and integrated and mean optical densities (IOD, MOD) and IOD histograms were compared. It was shown that (1) there was no significant variation in optical densities in cell imprints and aspirates whereas PS significantly (P less than 0.01) differed in both preparations; (2) there were significant differences of the optical densities between tissue sections and cytological preparations, either imprints or aspirates, likely due to randomly cut nuclei in tissue sections; and (3) there was a significant difference between the PS of tissue sections and aspirates but no significant difference between tissue sections and imprints. It is concluded that fine-needle aspiration constitutes a convenient method for cell sampling, reliable for the diagnosis of malignancies. However, it may not reflect the heterogeneity of cell subpopulations in tissue.
对127例乳腺癌进行了免疫细胞化学检测（ICA），使用单克隆抗雌激素受体（ER ICA）、抗孕激素受体（PR ICA）和单克隆抗体Ki67（Ki67 ICA）。免疫染色程序应用于冷冻组织切片、肿瘤印片和细针穿刺抽吸物，以比较在三种不同类型标本中检测到的抗原分布变化。用过氧化物酶 - 抗过氧化物酶、抗生物素蛋白 - 生物素 - 过氧化物酶复合物和碱性磷酸酶 - 抗碱性磷酸酶复合物检测到的阳性反应，通过一个称为SAMBA 200（SAMBA TITN，法国格勒诺布尔）的计算机图像分析系统进行评估。应用了专门为分析组织切片和细胞学标本而开发的应用程序。该系统可以对肿瘤中抗原位点的异质性进行多参数、准确、可靠、可重复和自动化的评估。比较了每种标志物的阳性细胞表面（PS）、积分光密度和平均光密度（IOD、MOD）以及IOD直方图。结果表明：（1）细胞印片和穿刺抽吸物中的光密度无显著差异，而两种标本中的PS均有显著差异（P < 0.01）；（2）组织切片与细胞学标本（印片或穿刺抽吸物）之间的光密度存在显著差异，这可能是由于组织切片中细胞核随机切割所致；（3）组织切片与穿刺抽吸物的PS之间存在显著差异，但组织切片与印片之间无显著差异。结论是，细针穿刺抽吸是一种方便的细胞采样方法，对恶性肿瘤的诊断可靠。然而，它可能无法反映组织中细胞亚群的异质性。
