Jheng Pei-Ru, Lu Kun-Ying, Yu Shu-Huei, Mi Fwu-Long
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
Department of Materials Science and Engineering, Vanung University, Chung-Li, Taiwan.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:402-12. doi: 10.1016/j.colsurfb.2015.09.032. Epub 2015 Sep 21.
Indocyanine green (ICG) is a FDA-approved near-infrared (NIR) cyanine dye used in medical diagnostics. However, the utility of ICG remains limited by its unstable optical property, and concentration-dependent aggregation and precipitation. A chitosan-arginine conjugate (CS-N-Arg) was developed to increase the stability of ICG in physiological buffer saline via formation of strong electrostatic interactions between ICG and CS-N-Arg. The CS-N-Arg/ICG complex prevented ICG from aggregation and precipitation, thus it could serve as a theranostic nanomaterial for image-guided photothermal cancer therapy. The CS-N-Arg/ICG NPs showed excellent photostability, clear fluorescent images, and rapid temperature rise under laser irradiation. Cell viability assay indicated that CS-N-Arg/ICG NPs could efficiently suppress the growth of doxorubicin (DOX) resistant breast cancer cell (MCF-7/ADR cells) under NIR photothermal treatments. In combination of DOX with CS-N-Arg/ICG NPs, a combined effect was observed in MCF-7/ADR breast cancer cells due to dual hyperthermia and chemical therapeutic effects. The present observations suggest that CS-N-Arg/ICG NPs can effectively deliver ICG molecules to MCF-7/ADR breast cancer cells and overcome DOX resistance in the cells by hyperthermia.
吲哚菁绿（ICG）是一种经美国食品药品监督管理局（FDA）批准的用于医学诊断的近红外（NIR）花青染料。然而，ICG的效用仍受其不稳定的光学性质以及浓度依赖性聚集和沉淀的限制。开发了一种壳聚糖-精氨酸共轭物（CS-N-Arg），通过在ICG和CS-N-Arg之间形成强静电相互作用来提高ICG在生理缓冲盐水中的稳定性。CS-N-Arg/ICG复合物可防止ICG聚集和沉淀，因此可作为用于图像引导光热癌症治疗的诊疗纳米材料。CS-N-Arg/ICG纳米颗粒在激光照射下表现出优异的光稳定性、清晰的荧光图像和快速升温。细胞活力测定表明，在近红外光热治疗下，CS-N-Arg/ICG纳米颗粒可有效抑制阿霉素（DOX）耐药乳腺癌细胞（MCF-7/ADR细胞）的生长。DOX与CS-N-Arg/ICG纳米颗粒联合使用时，由于双重热疗和化学治疗作用，在MCF-7/ADR乳腺癌细胞中观察到联合效应。目前的观察结果表明，CS-N-Arg/ICG纳米颗粒可有效地将ICG分子递送至MCF-7/ADR乳腺癌细胞，并通过热疗克服细胞中的DOX耐药性。
