Liu Ying-jia, Song Guo-hong, Zhang Chen
Zhonghua Nan Ke Xue. 2015 Aug;21(8):681-6.
To explore the possible pain mechanism of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).
The models of CP/CPPS were established in male Wistar rats by the autoimmune method. The paw withdrawal threshold (PWT) was detected using Von Frey filament. The expressions of the substance P and c-fos in the prostate and spinal L5-S2 segments were determined by immunohistochemistry followed by analysis of their correlation with CP/CPPS.
Compared with the control rats, the CP/CPPS models showed significantly decreased PWT (P < 0.05), remarkable prostatic inflammation, enlarged scope of lesions, and obvious interstitial lymphocytic infiltration (P < 0.05). Both the expressions of substance P and c-fos were markedly elevated in the prostate and spinal dorsal horn (L5-S2) of the rat models (P < 0.05), but the expression of substance P in the prostate exhibited no correlation with that in the spinal cord (r = 0.099, P = 0.338), nor did that of c-fos (r = 0.027, P = 0.454).
The upregulated expressions of substance P and c-fos in the spinal cord L5-S2 sections may be associated with the pain mechanism of CP/CPPS.
探讨慢性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）可能的疼痛机制。
采用自身免疫法在雄性Wistar大鼠中建立CP/CPPS模型。使用von Frey细丝检测爪部撤离阈值（PWT）。通过免疫组织化学法测定前列腺和脊髓L5-S2节段中P物质和c-fos的表达，并分析它们与CP/CPPS的相关性。
与对照大鼠相比，CP/CPPS模型的PWT显著降低（P<0.05），前列腺炎症明显，病变范围扩大，间质淋巴细胞浸润明显（P<0.05）。大鼠模型的前列腺和脊髓背角（L5-S2）中P物质和c-fos的表达均显著升高（P<0.05），但前列腺中P物质的表达与脊髓中的表达无相关性（r = 0.099，P = 0.338），c-fos的表达也无相关性（r = 0.027，P = 0.454）。
脊髓L5-S2节段中P物质和c-fos表达上调可能与CP/CPPS的疼痛机制有关。
