Hao Li-Ya, Liu Li, Bai Xian-Guang, Zheng Xin-Hua
Department of Basic Medical Science, Pingdingshan College, Pingdingshan, Henan 467000, China.
Zhonghua Nan Ke Xue. 2016 Jul;22(7):602-607.
To investigate the expressions of substance P (SP) and neurokinin-1 receptor (NK-1R) in the posterior horn of the L5－S2 spinal cord in the rat model of chronic nonbacterial prostatitis (CNP) at different time points of modeling.
Forty adult male SD rats were randomly divided into four groups of equal number, control, 45 d model, 60 d model, and 90 d model, and proteins were obtained from the prostatic tissue of another 30 rats. The CNP model was made by intraperitoneal injection of 0.5 ml DPT vaccineand intradermal injection of mixed solution of 1 ml prostatein extract and complete adjuvant at a 1∶1 ratio, while the control rats were injected with the same volume of normal saline. At 45, 60, and 90 days after modeling, we measured the paw withdrawal threshold (PWT) of the rats, determined the levels of TNF-α, IL-1β, IL-2, and IL-10 in the prostate tissue by ELISA, observed the histomorphological changes in the prostate by transmission electron and light microscopy, and detected the expressions of SP and NK1-R in the L5－S2 spinal cord by immunohistochemistry.
The model rats showed significantly increased sensitivity to pain, with remarkably lowered PWT at 45, 60, and 90 days after modeling. The levels of TNF-α, IL-1β, IL-2, and IL-10 in the prostate tissue were markedly elevated in the CNP models as compared with those in the controls (all P<0.05), most significantly at 90 days (all P<0.05). Immunohistochemistry showed that the expressions of SP and NK-1R were remarkably higher in the CNP model groups than in the control (all P<0.05), the highest at 90 days. Light microscopy revealed no inflammatory cell infiltration in the prostate tissue of the control rats, and obvious edema and increased lymphocytes were observed with the prolonged time of modeling.Transmission electron microscopy showed inflammatory changes in the prostate tissue of the model rats and that peritubular interstitial edema was most obvious at 90 days, with widened intervals between peritubular cells and the epithelial base and increased numbers of fibroblasts and collagen fibrils.
The synthesis of SP and the level of NK-1R were increased in the posterior horn of the L5－S2 spinal cord in the rat model of CNP.
研究慢性非细菌性前列腺炎（CNP）大鼠模型建模不同时间点L5－S2脊髓后角中P物质（SP）和神经激肽-1受体（NK-1R）的表达情况。
将40只成年雄性SD大鼠随机分为4组，每组数量相等，即对照组、45 d模型组、60 d模型组和90 d模型组，并从另外30只大鼠的前列腺组织中获取蛋白质。通过腹腔注射0.5 ml DPT疫苗和皮内注射1 ml前列腺素提取物与完全佐剂按1∶1比例混合的溶液制备CNP模型，而对照组大鼠注射相同体积的生理盐水。在建模后45、60和90天，测量大鼠的爪部缩足阈值（PWT），通过酶联免疫吸附测定法（ELISA）测定前列腺组织中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）和白细胞介素-10（IL-10）的水平，通过透射电子显微镜和光学显微镜观察前列腺的组织形态学变化，并通过免疫组织化学检测L5－S2脊髓中SP和NK1-R的表达。
模型大鼠对疼痛的敏感性显著增加，在建模后45、60和90天PWT明显降低。与对照组相比，CNP模型组前列腺组织中TNF-α、IL-1β、IL-2和IL-10的水平显著升高（均P<0.05），在90天时最为显著（均P<0.05）。免疫组织化学显示，CNP模型组中SP和NK-1R的表达明显高于对照组（均P<0.05），在90天时最高。光学显微镜显示对照组大鼠前列腺组织无炎性细胞浸润，随着建模时间延长，可见明显水肿和淋巴细胞增多。透射电子显微镜显示模型大鼠前列腺组织有炎性改变，90天时肾小管周围间质水肿最明显，肾小管细胞与上皮基底之间的间隙增宽，成纤维细胞和胶原纤维数量增加。
在CNP大鼠模型中，L5－S2脊髓后角中SP的合成及NK-1R的水平升高。
