三种检测产肠毒素大肠杆菌热稳定肠毒素检测系统的比较。
Comparison of three assay systems for detection of enterotoxigenic Escherichia coli heat-stable enterotoxin.
作者信息
Cryan B
机构信息
Department of Medical Microbiology, Regional Hospital, Galway, Ireland.
出版信息
J Clin Microbiol. 1990 Apr;28(4):792-4. doi: 10.1128/jcm.28.4.792-794.1990.
Abstract
In this study, a commercial DNA-DNA hybridization kit for the detection of Escherichia coli heat-stable enterotoxin is compared with a competitive enzyme-linked immunosorbent assay (ELISA) and the suckling mouse bioassay. Taking the suckling mouse assay as the "gold standard," the gene probe was the more specific and the ELISA was the more sensitive of the assays. The ELISA and the suckling mouse test are semiquantitative. The ELISA was the most rapid method, most amenable to automation, and most suitable for the examination of large numbers of specimens. The gene probe is particularly applicable in relatively primitive laboratory conditions. The suckling mouse assay was the least suitable system for the examination of large numbers of specimens.
摘要
在本研究中，将一种用于检测大肠杆菌热稳定肠毒素的商用DNA-DNA杂交试剂盒与竞争性酶联免疫吸附测定（ELISA）和乳鼠生物测定法进行了比较。以乳鼠测定法作为“金标准”，基因探针检测法特异性更强，而ELISA检测法灵敏度更高。ELISA和乳鼠检测法都是半定量的。ELISA是最快速的方法，最适合自动化操作，也最适合检测大量标本。基因探针特别适用于相对简陋的实验室条件。乳鼠测定法是最不适合检测大量标本的系统。
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2
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