Baba N, Baba M, Imamura M, Koga M, Ohsumi Y, Osumi M, Kanaya K
Department of Electrical Engineering, Kogakuin University, Shinjuku-ku, Japan.
J Electron Microsc Tech. 1989 Jan;11(1):16-26. doi: 10.1002/jemt.1060110104.
A computer graphics system for reconstruction from serial section micrographs was applied to intracellular details of a yeast target cell (Saccharomyces cerevisiae cell) induced by the alpha factor mating pheromone and was also applied to a periodontal structure of a dog tooth moved orthodontically. In the former, intracellular organelles and a distribution of vesicles could be clearly observed through the cell membrane using the transparent display method in which the smoothing of the reconstructed outer cell membrane surface by computer processing was applied to the transparent display. In the latter case, by cutting through a reconstructed dog tooth and its periodontal tissues, labiolingual and mesiodistal cut surfaces of the tooth and of adjacent alveolar bone could be observed with fine details (232 sections were used).
一种用于从连续切片显微照片进行重建的计算机图形系统，被应用于由α因子交配信息素诱导的酵母靶细胞（酿酒酵母细胞）的细胞内细节，也被应用于正畸移动的犬齿的牙周结构。在前者中，通过使用透明显示方法，即通过计算机处理对重建的细胞外膜表面进行平滑处理后应用于透明显示，能够透过细胞膜清晰观察到细胞内细胞器和囊泡的分布。在后者的情况下，通过切割重建的犬齿及其牙周组织，可以观察到牙齿和相邻牙槽骨的唇舌向和近远中向切割面的精细细节（使用了232个切片）。
