Bao Zekun, Gao Xue, Zhang Qiang, Lin Jian, Hu Weiwei, Yu Huiqing, Chen Jianquan, Yang Qian, Yu Qinghua
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Shanghai Transgenic Research Center, 88 Cai-Lun Road, Shanghai, 201210, People's Republic of China.
PLoS One. 2015 Oct 7;10(10):e0139822. doi: 10.1371/journal.pone.0139822. eCollection 2015.
The development of genetically engineered animals has brought with it increasing concerns about biosafety issues. We therefore evaluated the risks of growth hormone from transgenic goats, including the probability of horizontal gene transfer and the impact on the microbial community of the goats' gastrointestinal tracts, feces and the surrounding soil. The results showed that neither the GH nor the neoR gene could be detected in the samples. Moreover, there was no significant change in the microbial community of the gastrointestinal tracts, feces and soil, as tested with PCR-denaturing gradient gel electrophoresis and 16S rDNA sequencing. Finally, phylogenetic analysis showed that the intestinal content, feces and soil samples all contained the same dominant group of bacteria. These results demonstrated that expression of goat growth hormone in the mammary of GH transgenic goat does not influence the microflora of the intestine, feces and surrounding soil.
基因工程动物的发展引发了人们对生物安全问题的日益关注。因此，我们评估了转基因山羊生长激素的风险，包括水平基因转移的可能性以及对山羊胃肠道、粪便和周围土壤微生物群落的影响。结果表明，在样本中未检测到生长激素（GH）基因和新霉素抗性（neoR）基因。此外，通过PCR-变性梯度凝胶电泳和16S rDNA测序检测发现，胃肠道、粪便和土壤中的微生物群落没有显著变化。最后，系统发育分析表明，肠道内容物、粪便和土壤样本中都含有相同的优势菌群。这些结果表明，GH转基因山羊乳腺中表达的山羊生长激素不会影响肠道、粪便和周围土壤中的微生物群落。
