Basharat Zarrin, Yasmin Azra
Microbiology and Biotechnology Research Laboratory, Department of Environmental Sciences, Fatima Jinnah Women University 46000, Pakistan.
Can J Microbiol. 2015 Dec;61(12):898-902. doi: 10.1139/cjm-2015-0136. Epub 2015 Aug 31.
Distinct simple sequence repeats with 2 or more individual microsatellites joined together or lying adjacent to each other are identified as compound microsatellites. Investigation of such composite microsatellites in the genomes of genus Lactobacillus was the aim of this study. In silico inspection of microsatellite clustering in genomes of 14 Lactobacillus species revealed a wealth of compound microsatellites. All of the mined compound microsatellites were imperfect, were composed of variant motifs, and increased in all genomes, with maximum distance (dMAX) increments of 10 to 50. The majority of these repeats were present in the coding regions. A correlation of microsatellite to compound microsatellite density was detected. The difference established in compound microsatellite division among eukaryotes, Escherichia coli, and lactobacilli is suggestive of diverse genomic features and elementary distinction between creation and fixation methods of compound microsatellites among these organisms.
具有2个或更多个单独微卫星连接在一起或彼此相邻排列的独特简单序列重复被鉴定为复合微卫星。本研究的目的是对乳酸杆菌属基因组中的此类复合微卫星进行研究。对14种乳酸杆菌物种基因组中的微卫星聚类进行电子检查，发现了大量的复合微卫星。所有挖掘出的复合微卫星都是不完美的，由变异基序组成，并且在所有基因组中都有所增加，最大距离（dMAX）增量为10至50。这些重复序列大多存在于编码区域。检测到微卫星与复合微卫星密度之间的相关性。真核生物、大肠杆菌和乳酸杆菌在复合微卫星划分上的差异表明了不同的基因组特征以及这些生物体中复合微卫星产生和固定方法的基本区别。
