Sluimer Judith C, Gijbels Marion J, Heeneman Sylvia
Department of Pathology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, 5800, 6202 AZ, The Netherlands.
Department of Molecular Genetics, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands.
Methods Mol Biol. 2015;1339:339-48. doi: 10.1007/978-1-4939-2929-0_24.
Intraplaque hemorrhage is defined as the presence of fresh or lysed erythrocytes, iron deposits in macrophages, and/or a fibrin clot in an atherosclerotic plaque. These features can be detected by hematoxylin and eosin, Martius scarlet Blue, and Perl's iron histological stainings. It is noteworthy that intraplaque hemorrhage is only present in murine atherosclerotic plaques after additional interventions or additional genetic traits affecting matrix degradation or thrombosis. In this chapter, we describe methods to detect intraplaque hemorrhage in mouse atherosclerotic lesions.
斑块内出血的定义为动脉粥样硬化斑块中存在新鲜或溶解的红细胞、巨噬细胞中的铁沉积和/或纤维蛋白凝块。这些特征可通过苏木精和伊红染色、马休黄猩红蓝染色以及佩尔铁染色法检测到。值得注意的是，只有在进行额外干预或存在影响基质降解或血栓形成的额外遗传特征后，小鼠动脉粥样硬化斑块中才会出现斑块内出血。在本章中，我们描述了检测小鼠动脉粥样硬化病变中斑块内出血的方法。
