Andrés-Manzano M Jesús, Andrés Vicente, Dorado Beatriz
Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Vascular Biology Program, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029, Madrid, Spain.
Methods Mol Biol. 2015;1339:85-99. doi: 10.1007/978-1-4939-2929-0_5.
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.
用油红O和苏木精-伊红对组织进行染色的方法是经典的组织学技术，因其使用方便、可靠性高以及能提供大量信息，而被广泛用于量化小鼠组织中的动脉粥样硬化负担。这些染色方法可以提供有关遗传或环境因素对动脉粥样硬化负担以及疾病的起始、进展或消退影响的定量数据，还可用于评估旨在预防或治疗动脉粥样硬化的药物的疗效。本章提供了量化小鼠主动脉和主动脉根部动脉粥样硬化负担的方案，包括解剖、油红O染色、苏木精-伊红染色和图像分析的方法。
