Chèvre Raphaël
Department of Atherothrombosis, Imaging and Epidemiology, CNIC (Spanish National Cardiovascular Research Center), C/Melchor Fernández Almagro 3, 28029, Madrid, Spain.
Methods Mol Biol. 2015;1339:349-55. doi: 10.1007/978-1-4939-2929-0_25.
We present here a procedure that allows real-time high-resolution multichannel imaging of early atherosclerotic lesions of live mice, by dramatically reducing the respiratory and pulsatile movements of the athero-susceptible carotid artery, without significantly altering blood flow dynamics. This surgical preparation can be combined with the use of various fluorescent probes and reporter mice to simultaneously visualize the dynamics of inflammatory leukocytes, platelets, or even subcellular structures. Stabilization of the tissue renders it suitable for two-photon laser scanning microscopic imaging and allows tracking the behavior of inflammatory cells in three dimensions.
我们在此介绍一种方法，该方法通过显著减少易患动脉粥样硬化的颈动脉的呼吸和搏动运动，同时又不显著改变血流动力学，从而实现对活体小鼠早期动脉粥样硬化病变的实时高分辨率多通道成像。这种手术准备可与使用各种荧光探针和报告基因小鼠相结合，以同时可视化炎症白细胞、血小板甚至亚细胞结构的动态变化。组织的稳定使其适用于双光子激光扫描显微镜成像，并允许在三维空间中追踪炎症细胞的行为。
