Arranz Alicia, Rudin Markus, Zaragoza Carlos, Ripoll Jorge
Institute for Biomedical Engineering, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 10, 8093, Zurich, Switzerland.
Cardiovascular Research Unit, Hospital Ramón y Cajal, Universidad Francisco de Vitoria, 28223 Pozuelo de Alarcón, Madrid, Spain.
Methods Mol Biol. 2015;1339:367-76. doi: 10.1007/978-1-4939-2929-0_27.
Optical imaging technologies such as fluorescence molecular tomography (FMT) are gaining great relevance in cardiovascular research. The main reason is the increased number of available fluorescent agents, especially those termed "activatable probes," which remain quenched under baseline conditions and are fluorescent when a specific enzymatic activity is present. A major characteristic of FMT is the possibility of obtaining quantitative data of fluorescence signal distribution in a noninvasive fashion and using nonionizing radiation, making FMT an invaluable tool for longitudinal studies with biomedical applications. Here, we describe a standard procedure to perform FMT experiments in atherosclerosis mouse models, from the handling of the animals to the reconstruction of the 3D images.
诸如荧光分子断层扫描（FMT）等光学成像技术在心血管研究中越来越重要。主要原因是可用荧光剂的数量增加，特别是那些被称为“可激活探针”的荧光剂，它们在基线条件下保持淬灭状态，而当存在特定酶活性时会发出荧光。FMT的一个主要特点是能够以无创方式获得荧光信号分布的定量数据，并使用非电离辐射，这使得FMT成为生物医学应用纵向研究中一个非常有价值的工具。在这里，我们描述了在动脉粥样硬化小鼠模型中进行FMT实验的标准程序，从动物的处理到三维图像的重建。
