利用对比剂的荧光分子断层成像术无创可视化阿尔茨海默病淀粉样变小鼠中的淀粉样-β 沉积
Noninvasive Visualization of Amyloid-Beta Deposits in Alzheimer's Amyloidosis Mice via Fluorescence Molecular Tomography Using Contrast Agent.
机构信息
School of Information Science and Technology, ShanghaiTech University, Shanghai, China.
Institute for Regenerative Medicine, University of Zurich, Zurich, Switzerland.
出版信息
Methods Mol Biol. 2024;2785:271-285. doi: 10.1007/978-1-0716-3774-6_16.
Alzheimer's disease is pathologically featured by the accumulation of amyloid-beta (Aβ) plaque and neurofibrillary tangles. Compared to small animal positron emission tomography, optical imaging features nonionizing radiation, low cost, and logistic convenience. Optical detection of Aβ deposits is typically implemented by 2D macroscopic imaging and various microscopic techniques assisted with Aβ-targeted contrast agents. Here, we introduce fluorescence molecular tomography (FMT), a macroscopic 3D fluorescence imaging technique, convenient for in vivo longitudinal monitoring of the animal brain without the involvement of cranial window opening operation. This chapter aims to provide the protocols for FMT in vivo imaging of Aβ deposits in the brain of rodent model of Alzheimer's disease. The materials, stepwise method, notes, limitations of FMT, and emerging opportunities for FMT techniques are presented.
阿尔茨海默病的病理学特征是淀粉样β（Aβ）斑块和神经原纤维缠结的积累。与小动物正电子发射断层扫描相比，光学成像是一种非电离辐射，成本低，物流方便。Aβ 沉积物的光学检测通常通过 2D 宏观成像和各种微观技术与 Aβ 靶向对比剂辅助来实现。在这里，我们介绍荧光分子断层扫描（FMT），这是一种宏观 3D 荧光成像技术，便于在不进行颅窗开口操作的情况下对动物大脑进行体内纵向监测。本章旨在提供 FMT 在阿尔茨海默病啮齿动物模型大脑中 Aβ 沉积物体内成像的方案。介绍了 FMT 的材料、逐步方法、注意事项、局限性以及 FMT 技术的新机遇。
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