聚集诱导发光分子掺入聚合物纳米颗粒作为 FRET 供体以观察阿霉素的递送。
Aggregation-Induced-Emissive Molecule Incorporated into Polymeric Nanoparticulate as FRET Donor for Observing Doxorubicin Delivery.
机构信息
School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology , Tianjin 300384, China.
Department of Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
出版信息
ACS Appl Mater Interfaces. 2015 Oct 28;7(42):23760-6. doi: 10.1021/acsami.5b08202. Epub 2015 Oct 15.
Tetraphenylethene (TPE) derivatives characterized with distinct aggregation-induced-emission, attempted to aggregate with doxorubicin (Dox) to formulate the interior compartment of polymeric nanoparticulate, served as fluorescence resonance energy transfer (FRET) donor to promote emission of acceptor Dox. Accordingly, this FRET formulation allowed identification of Dox in complexed form by detecting FRET. Important insight into the Dox releasing can be subsequently explored by extracting complexed Dox (FRET) from the overall Dox via direct single-photon excitation of Dox. Of note, functional catiomers were used to complex with FRET partners for a template formulation, which was verified to induce pH-responsive release in the targeted subcellular compartment. Hence, this well-defined multifunctional system entitles in situ observation of the drug releasing profile and insight on drug delivery journey from the tip of injection vein to the subcellular organelle of the targeted cells.
具有明显聚集诱导发光特性的四苯乙烯（TPE）衍生物，试图与阿霉素（Dox）聚集，形成聚合物纳米颗粒的内部隔室，作为荧光共振能量转移（FRET）供体，以促进受体 Dox 的发射。因此，这种 FRET 制剂可以通过检测 FRET 来识别复合物形式的 Dox。通过直接单光子激发 Dox 从总 Dox 中提取复合物 Dox（FRET），可以进一步深入了解 Dox 的释放情况。值得注意的是，功能型正离子用于与 FRET 供体复合物形成模板制剂，该制剂被证实可在靶向亚细胞区室中诱导 pH 响应性释放。因此，这种定义明确的多功能系统可以在原位观察药物释放情况，并深入了解从注射静脉尖端到靶向细胞亚细胞器的药物输送过程。
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