1 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education P.R. China, Inner Mongolia Agricultural University, Inner Mongolia, Hohhot 010018, China P.R.
Benef Microbes. 2016 Feb;7(1):95-102. doi: 10.3920/BM2015.0048. Epub 2015 Oct 9.
Bovine mastitis is an economical important microbial disease in dairy industry. Some recent human clinical trials have shown that oral probiotics supplementation could effectively control clinical mastitis, suggesting that the mechanism of mastitis protection might be achieved via the host gut microbiota. We aimed to test our hypothesis that bovine mastitis was related to changes in both the mammary and gut microbial profiles. By quantitative PCR, the milk and faecal microbial profiles of cows with low (<3×10 cells/ml) and high (>1×10 cells/ml) somatic cell count (SCC) were compared. Firstly, we observed drastic differences in both the milk and faecal microbial compositions at genus and Lactobacillus-species levels between the two groups. Secondly, the pattern of faecal microbial community changes of mastitis cows was similar to that of the milk, characterised by a general increase in the mastitis pathogens (Enterococcus, Streptococcus and Staphylococcus) and deprivation of Lactobacillus and its members (L. salivarius, L. sakei, L. ruminis, L. delbrueckii, L. buchneri, and L. acidophilus). Thirdly, only the faecal lactobacilli, but not bifidobacteria correlated with the milk microbial communities and SCC. Our data together hint to a close association between bovine mastitis, the host gut and milk microbiota.
奶牛乳腺炎是奶牛养殖业中一种具有重要经济意义的微生物疾病。一些最近的人类临床试验表明，口服益生菌补充剂可以有效地控制临床乳腺炎，这表明乳腺炎保护的机制可能是通过宿主肠道微生物群来实现的。我们旨在检验我们的假设，即奶牛乳腺炎与乳腺和肠道微生物群的变化有关。通过定量 PCR，比较了体细胞计数（SCC）低（<3×10 细胞/ml）和高（>1×10 细胞/ml）的奶牛的奶和粪便微生物群。首先，我们在属和乳杆菌种水平上观察到两组之间的奶和粪便微生物组成存在明显差异。其次，乳腺炎奶牛粪便微生物群落变化的模式与奶相似，其特征是乳腺炎病原体（肠球菌、链球菌和葡萄球菌）普遍增加，而乳杆菌及其成员（唾液乳杆菌、清酒乳杆菌、发酵乳杆菌、德氏乳杆菌、布氏乳杆菌和嗜酸乳杆菌）减少。第三，只有粪便中的乳杆菌，而不是双歧杆菌与奶微生物群和 SCC 相关。我们的数据共同提示奶牛乳腺炎与宿主肠道和奶微生物群密切相关。
