González-Fernández Rebeca, Hernández Jairo, Martín-Vasallo Pablo, Puopolo Maria, Palumbo Angela, Ávila Julio
Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular, Universidad de la Laguna, La Laguna, Spain.
Centro de Asistencia a la Reproducción Humana de Canarias, La Laguna, Spain.
Reprod Sci. 2016 May;23(5):604-9. doi: 10.1177/1933719115607996. Epub 2015 Oct 8.
Oxidative stress (OS) plays an important role in all physiological processes. The effect of OS on cellular processes is modulated by the ability of the cell to express genes implicated in the reversal of lipid, protein, and DNA injury. Aldehyde dehydrogenase 3, member A2 (ALDH3A2) is a ubiquitous enzyme involved in lipid detoxification. The objective of this study was to investigate the expression ofALDH3A2in human granulosa-lutein (GL) cells of women undergoing in vitro fertilization (IVF) and its relationship with age, infertility diagnosis, and IVF outcome variables. Relative expression levels ofALDH3A2were determined by quantitative reverse transcription-polymerase chain reaction. To investigate the effect of age onALDH3A2expression, 72 women between 18 and 44 years of age with no ovarian factor (NOF) were analyzed. To evaluate the effect of infertility diagnosis onALDH3A2expression, the following groups were analyzed: 22 oocyte donors (ODs), 24 women >40 years old (yo) with tubal or male factor and no ovarian pathology, 18 poor responders (PRs), 19 cases with endometriosis (EM), and 18 patients with polycystic ovarian syndrome (PCOS). In NOF,ALDH3A2expression correlated positively with age and with the doses of follicle-stimulating hormone and luteinizing hormone administered and negatively with the number of total and mature oocytes. When different groups were analyzed,ALDH3A2expression levels were higher in patients >40 yo and in PR compared to OD. On the contrary, EM and PCOS levels were lower than expected for age. These data suggest that GL cellALDH3A2expression levels correlate with age, cause of infertility, and ovarian response to stimulation.
氧化应激（OS）在所有生理过程中都起着重要作用。细胞表达与脂质、蛋白质和DNA损伤逆转相关基因的能力调节了OS对细胞过程的影响。醛脱氢酶3家族成员A2（ALDH3A2）是一种参与脂质解毒的普遍存在的酶。本研究的目的是调查接受体外受精（IVF）的女性人颗粒黄体（GL）细胞中ALDH3A2的表达及其与年龄、不孕诊断和IVF结局变量的关系。通过定量逆转录-聚合酶链反应测定ALDH3A2的相对表达水平。为了研究年龄对ALDH3A2表达的影响，分析了72名年龄在18至44岁之间且无卵巢因素（NOF）的女性。为了评估不孕诊断对ALDH3A2表达的影响，分析了以下几组：22名卵母细胞捐赠者（ODs）、24名年龄>40岁（yo）且有输卵管或男性因素且无卵巢病变的女性、18名低反应者（PRs）、19名子宫内膜异位症（EM）患者和18名多囊卵巢综合征（PCOS）患者。在NOF中，ALDH3A2表达与年龄、促卵泡生成素和促黄体生成素的给药剂量呈正相关，与总卵母细胞和成熟卵母细胞数量呈负相关。当分析不同组时，与OD相比，年龄>40岁的患者和PR中的ALDH3A2表达水平更高。相反，EM和PCOS水平低于预期年龄。这些数据表明，GL细胞ALDH3A2表达水平与年龄、不孕原因和卵巢对刺激的反应相关。
