光活化底物用于干细胞的特异性分化。
Photo-Activatable Substrates for Site-Specific Differentiation of Stem Cells.
机构信息
Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University , Wuhan 430072, China.
College of Science, Huazhong Agricultural University , Wuhan 430070, China.
出版信息
ACS Appl Mater Interfaces. 2015 Oct 28;7(42):23679-84. doi: 10.1021/acsami.5b07455. Epub 2015 Oct 19.
In this report, a UV sensitive, PEGylated PFSSTKTC (Pro-Phe-Ser-Ser-Thr-Lys-Thr-Cys) peptide was modified on quartz substrate to investigate the spatial controlled differentiation of stem cells. This substrate could restrict the cell adhesion due to the steric hindrance of PEG shell. With UV irradiation, PFSSTKTC became exposed owing to the breakage of o-nitrobenzyl group with the detachment of PEG shell. The irradiation boundary on substrate was stable in the long term. The in vitro osteogenic differentiation results revealed that under the site-specific irradiation, the mesenchymal stem cells (MSCs) could specifically differentiate into osteoblast under the induction of PFSSTKTC peptide. This photoactivatable biomaterial shows great potential for region controllable and precise MSCs differentiation.
在本报告中，一种对紫外线敏感的、聚乙二醇（PEG）化的 PFSSTKTC（脯氨酸-苯丙氨酸-丝氨酸-苏氨酸-赖氨酸-苏氨酸-半胱氨酸）肽被修饰在石英基底上，以研究干细胞的空间控制分化。由于 PEG 壳的空间位阻，这种基底可以限制细胞的黏附。随着紫外线的照射，由于邻硝基苄基的断裂和 PEG 壳的脱落，PFSSTKTC 变得暴露出来。基底上的辐照边界在长时间内保持稳定。体外成骨分化结果表明，在特定部位的辐照下，间充质干细胞（MSCs）在 PFSSTKTC 肽的诱导下可以特异性地分化为成骨细胞。这种光激活生物材料显示出在区域可控和精确的 MSC 分化方面的巨大潜力。
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