真空紫外光处理对钛基表面光功能化后对成骨细胞行为的评价。
Evaluation of osteoblastic cell behavior upon culture on titanium substrates photo-functionalized by vacuum ultra-violet treatment.
机构信息
Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami Chuo-ku, Kumamoto, 860-8555, Japan; International Research Organization for Advanced Science & Technology, Kumamoto University, 2-39-1 Kurokami Chuo-ku, Kumamoto, 860-8555, Japan; Institute of Industrial Nanomaterials, 2-39-1 Kurokami Chuo-ku, Kumamoto, 860-8555, Japan.
Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, 860-8555, Japan.
出版信息
Exp Cell Res. 2022 Jan 1;410(1):112944. doi: 10.1016/j.yexcr.2021.112944. Epub 2021 Nov 22.
Photo-functionalization of titanium orthopedic/prosthetic implants using ultraviolet illumination is known to improve osteogenesis. Therefore, in this study, we aimed to examine the influence of vacuum ultraviolet (VUV)-treated titanium surfaces on osteoblast cell adhesion, activity, and differentiation. Osteoblastic cells were cultured on titanium substrates treated with various VUV treatment conditions (0, 6.2, 18.7, and 37.4 J/cm) and their behavior was evaluated. The results revealed that cell adhesion was increased whereas cell activity and differentiation ability were decreased upon cell culture on VUV-treated substrates. In particular, cell activity and differentiation ability were dramatically suppressed with 18.7 J/cm VUV irradiation. Within the limitations of this cell-based experiment, we clarified the VUV treatment conditions in which cell adhesion was improved but cell activity and differentiation ability were suppressed. These results indicate that VUV-treatment can be used to influence cell growth properties and can be used to accelerate or suppress cell differentiation on implant substrates.
使用紫外线照射对骨科/假体植入物进行光功能化已被证明可以促进成骨。因此，在这项研究中，我们旨在研究真空紫外线（VUV）处理的钛表面对成骨细胞黏附、活性和分化的影响。将成骨细胞培养在经过不同 VUV 处理条件（0、6.2、18.7 和 37.4 J/cm）处理的钛基底上，并评估其行为。结果表明，细胞黏附在 VUV 处理的基底上培养时增加，而细胞活性和分化能力降低。特别是，用 18.7 J/cm VUV 照射时，细胞活性和分化能力显著受到抑制。在这个基于细胞的实验的限制内，我们阐明了 VUV 处理条件，在这些条件下，细胞黏附得到改善，但细胞活性和分化能力受到抑制。这些结果表明，VUV 处理可以用来影响细胞的生长特性，并可用于加速或抑制植入物基底上的细胞分化。
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