一种用于灵敏检测尿酸的基于金纳米簇的传感器。
A gold nanocluster-based sensor for sensitive uric acid detection.
作者信息
Xu Pingping, Li Ruiping, Tu Yifeng, Yan Jilin
机构信息
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Suzhou University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Suzhou University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
出版信息
Talanta. 2015 Nov 1;144:704-9. doi: 10.1016/j.talanta.2015.07.027. Epub 2015 Jul 10.
In this report, we developed a highly sensitive and selective sensor for the detection of uric acid. Gold nanoclusters were synthesized with bovine serum albumin as the template material. Under the catalysis of urate oxidase, hydrogen peroxide was generated, which quenched the fluorescence from the gold nanoclusters. Furthermore, excessive iodide was found capable of enhancing this quenching effect, which significantly improved the sensitivity of the detection. Under an optimized condition, the extent of quenching was found linearly related to uric acid concentration in the range of 0.7-80 μM, and uric acid as low as 120 nM could be detected. With simple dilutions, blood samples could be analyzed, and satisfactory recoveries were obtained.
在本报告中，我们开发了一种用于检测尿酸的高灵敏度和高选择性传感器。以牛血清白蛋白为模板材料合成了金纳米簇。在尿酸氧化酶的催化下，产生了过氧化氢，其淬灭了金纳米簇发出的荧光。此外，发现过量的碘离子能够增强这种淬灭效果，从而显著提高了检测的灵敏度。在优化的条件下，发现淬灭程度与0.7 - 80 μM范围内的尿酸浓度呈线性关系，并且能够检测低至120 nM的尿酸。通过简单稀释即可对血样进行分析，并获得了令人满意的回收率。
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