Hao Shaodong, Wang He, Tao Wanqiang, Wang Jinzhong, Zhang Zhiyong, Zhang Qiuling, Zhang Minzhao, Guo Li, Shi Xiaoyu
Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
Beijing Forest Protection Station,Beijing 100029, China.
J Econ Entomol. 2015 Oct;108(5):2443-9. doi: 10.1093/jee/tov191. Epub 2015 Jul 3.
The insect family Cicadellidae includes economically important vectors of plant pathogens. Hishimonus sellatus (Uhler) transmits jujube witches'-broom (JWB). Currently, H. sellatus and Hishimonus lamellatus Cai et Kuoh are observed to co-occur at the same locality on jujube. H. lamellatus is now suspected to be a JWB vector. As such, correct identification of Hishimonus species present in vineyards is essential for epidemiological surveys. However, traditional identification of Hishimonus by morphology is limited to the adult male. We provide a comprehensive description of morphological and molecular tools for discriminating between H. sellatus and H. lamellatus, for use in identification and monitoring of the two Hishimonus species and studies of their plant hosts. A rapid and inexpensive method is introduced to identify H. sellatus and H. lamellatus occurring in jujube orchards. This method is based on amplification of mitochondrial cytochrome oxidase I (COI) gene, using PCR with multiplexed, species-specific primers. The reliability of this new method has been tested on different populations from different sites in Beijing region of China.
叶蝉科昆虫包含对经济有重要影响的植物病原体传播媒介。桑拟菱纹叶蝉（Hishimonus sellatus (Uhler)）传播枣疯病（JWB）。目前，观察到桑拟菱纹叶蝉和蔡氏拟菱纹叶蝉（Hishimonus lamellatus Cai et Kuoh）在枣树上的同一地点共存。现在怀疑蔡氏拟菱纹叶蝉也是枣疯病的传播媒介。因此，准确鉴定葡萄园中的拟菱纹叶蝉种类对于流行病学调查至关重要。然而，传统的通过形态学对拟菱纹叶蝉进行鉴定仅限于成年雄性。我们提供了用于区分桑拟菱纹叶蝉和蔡氏拟菱纹叶蝉的形态学和分子工具的全面描述，并将其用于这两种拟菱纹叶蝉的鉴定、监测以及它们植物寄主的研究。介绍了一种快速且低成本的方法来鉴定枣园中出现的桑拟菱纹叶蝉和蔡氏拟菱纹叶蝉。该方法基于使用多重物种特异性引物进行PCR扩增线粒体细胞色素氧化酶I（COI）基因。这种新方法的可靠性已在中国北京地区不同地点的不同种群上进行了测试。
