Zhuang Junjun, Lin Jun, Li Juan, Weng Wenjian, Cheng Kui, Wang Huiming
School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:479-87. doi: 10.1016/j.colsurfb.2015.09.050. Epub 2015 Sep 30.
Mineralized collagen coatings were synthesized by electrochemical deposition with alternating negative and positive potentials. The obtained coatings demonstrated a multi-layer structure alternating consisting of weakly and highly mineralized collagen layers and the proportion of each layer could be controlled by adjusting the deposition time. The coatings deposited using alternating potentials assisted electrochemical deposition (AP-ECD) showed significantly enhanced osteoblasts proliferation, and rhBMP-2 loading capability compared to those of the coatings deposited using constant potential electrochemical deposition (CP-ECD). The enhanced cytocompatibility and rhBMP-2 loading capability of the coatings might be attributed to their high proportion of weakly mineralized collagen layer. Furthermore, the deposition mechanism for alternating potentials is proposed as that positive potential induces deposition of negatively charged collagen fibrils to form a weakly mineralized collagen layer. Our results suggest that the present deposition method could be a promising approach to engineer mineralized collagen coating with better biological performances.
通过交替施加负电位和正电位的电化学沉积法合成了矿化胶原涂层。所获得的涂层呈现出由弱矿化胶原层和强矿化胶原层交替组成的多层结构，并且每层的比例可通过调整沉积时间来控制。与使用恒电位电化学沉积（CP-ECD）制备的涂层相比，采用交替电位辅助电化学沉积（AP-ECD）制备的涂层显示出显著增强的成骨细胞增殖能力和重组人骨形态发生蛋白-2（rhBMP-2）负载能力。涂层增强的细胞相容性和rhBMP-2负载能力可能归因于其高比例的弱矿化胶原层。此外，提出交替电位的沉积机制为正电位诱导带负电荷的胶原原纤维沉积形成弱矿化胶原层。我们的结果表明，目前的沉积方法可能是一种制备具有更好生物学性能的矿化胶原涂层的有前景的方法。
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