Wee Eugene J H, Ha Ngo Thu, Trau Matt
Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St Lucia, Queensland, Australia.
School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland, Australia.
Sci Rep. 2015 Oct 13;5:15028. doi: 10.1038/srep15028.
The challenge of bringing DNA methylation biomarkers into clinic is the lack of simple methodologies as most current assays have been developed for research purposes. To address the limitations of current methods, we describe herein a novel methyl-protein domain (MBD) enrichment protocol for simple yet rapid and highly stringent selection of highly methylated DNA from limiting input samples. We then coupled this with a DNA-mediated flocculation assay for rapid and low cost naked-eye binary evaluation of highly methylated genes in cell line and blood DNA. The low resource requirements of our method may enable widespread adoption of DNA methylation-based diagnostics in clinic and may be useful for small-scale research.
将DNA甲基化生物标志物应用于临床面临的挑战是缺乏简单的方法，因为目前大多数检测方法都是为研究目的而开发的。为了解决现有方法的局限性，我们在此描述了一种新型甲基化蛋白结构域（MBD）富集方案，用于从有限的输入样本中简单、快速且高度严格地筛选高度甲基化的DNA。然后，我们将其与DNA介导的絮凝检测相结合，用于对细胞系和血液DNA中的高度甲基化基因进行快速、低成本的肉眼二元评估。我们的方法对资源要求较低，可能会使基于DNA甲基化的诊断方法在临床上得到广泛应用，并且可能对小规模研究有用。
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