Department of Parasitology, Faculty of Medicine Universitas Brawijaya, Malang, Indonesia.
Malaria Research Group, Faculty of Medicine Universitas Brawijaya, Malang, Indonesia.
J Clin Lab Anal. 2022 Apr;36(4):e24314. doi: 10.1002/jcla.24314. Epub 2022 Mar 4.
Update diagnostic methods play essential roles in dealing with the current global malaria situation and decreasing malaria incidence.
Global malaria control programs require the availability of adequate laboratory tests in the quick and convenient field.
There are several methods to find out the existence of parasites within the blood. The oldest one is by microscopy, which is still a gold standard, although rapid diagnostic tests (RDTs) have rapidly become a primary diagnostic test in many endemic areas. Because of microscopy and RDTs limitation, novel serological and molecular methods have been developed. Many kinds of polymerase chain reaction (PCR) provide rapid results and higher specificity and sensitivity. The loop-mediated isothermal amplification (LAMP) and biosensing-based molecular techniques as point of care tests (POCT) will become a cost-effective approach to advance diagnostic testing.
Despite conventional techniques are still being used in the field, the exploration and field implementation of advanced techniques for the diagnosis of malaria are still being developed rapidly.
更新诊断方法在应对当前全球疟疾形势和降低疟疾发病率方面发挥着重要作用。
全球疟疾控制项目需要在快速便捷的现场提供足够的实验室检测。
有几种方法可以发现血液中的寄生虫。最古老的方法是显微镜检查，尽管快速诊断检测（RDT）已经迅速成为许多流行地区的主要诊断检测方法，但它仍然是金标准。由于显微镜检查和 RDT 的局限性，已经开发出了新型的血清学和分子方法。许多种聚合酶链反应（PCR）提供快速结果以及更高的特异性和敏感性。环介导等温扩增（LAMP）和基于生物传感的分子技术作为即时检测（POCT）将成为推进诊断检测的具有成本效益的方法。
尽管常规技术仍在现场使用，但疟疾诊断先进技术的探索和现场实施仍在迅速发展。
