阿片肽-12肽及其结构类似物的体外抗氧化作用

Antioxidant Action of Apelin-12 Peptide and Its Structural Analog In Vitro.

作者信息

Pelogeykina Y A, Konovalova G G, Pisarenko O I, Lankin V Z

机构信息

Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2015 Sep;159(5):604-6. doi: 10.1007/s10517-015-3024-4. Epub 2015 Oct 13.

DOI:10.1007/s10517-015-3024-4

PMID:26459481

Abstract

The effects of C-terminal fragment of natural peptide apelin-12 H-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe-OH (A12) and its structural analog H-(N(α)Me)Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Nle-Pro-Phe-OH (AI) on Cu(2+)-induced free radical oxidation of low-density lipoprotein in human blood plasma and activity of commercially available enzymes superoxide dismutase and catalase in a concentration range of 0.01-1 mM were examined. A12 and AI had no effect on superoxide dismutase and catalase activities during 24-h co-incubation with these enzymes at 4°C. When used in a concentration of 1 mM, A12 and AI decreased the maximum low-density lipoprotein oxidation rate by 51 and 47%, respectively, and lengthened the lag phase of low-density lipoprotein oxidation by 2.6 and 1.8 times, respectively, which confirmed their antioxidant potency.

摘要

研究了天然肽apelin-12的C端片段H-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe-OH（A12）及其结构类似物H-(N(α)Me)Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Nle-Pro-Phe-OH（AI）在0.01-1 mM浓度范围内对人血浆中铜（2+）诱导的低密度脂蛋白自由基氧化以及市售超氧化物歧化酶和过氧化氢酶活性的影响。在4°C下与这些酶共同孵育24小时期间，A12和AI对超氧化物歧化酶和过氧化氢酶活性没有影响。当以1 mM的浓度使用时，A12和AI分别使低密度脂蛋白的最大氧化速率降低了51%和47%，并分别使低密度脂蛋白氧化的延迟期延长了2.6倍和1.8倍，这证实了它们的抗氧化能力。