Xu Jinhong, Yang Heping, Zhou Xiangdong, Wang Haijing, Gong Liang, Tang Chunlan
Department of Respiratory Medicine, Southwest Hospital, The Third Military Medical University, Chongqing 400038, P.R. China.
Mol Med Rep. 2015 Nov;12(5):7603-8. doi: 10.3892/mmr.2015.4356. Epub 2015 Sep 24.
Curcumin is an active component of the medicinal plant turmeric, which has been reported to have anti‑metastatic activities and induce autophagy in numerous cancer types. Bisdemethoxycurcumin (BDMC), one of the major active curcumin derivatives present in turmeric, was previously shown to trigger autophagy in highly metastatic large‑cell lung cancer 95D cells. However, the effects of the induction of autophagy by BDMC on the invasion and migration of 95D cells has remained elusive. Therefore, the present study investigated the effects of BDMC on the invasion and migration of highly metastatic large‑cell lung cancer 95D cells. Meanwhile we observed the effect of autophagy induced by BDMC on the migration and invasion in 95D cells. Transwell assays showed that BDMC exerted an inhibitory effect on the migration and invasion of 95D cells. Furthermore, the expression of vimentin was downregulated, while E‑cadherin expression was upregulated in 95D cells treated with BDMC. In addition, blockage of autophagy through Beclin1‑targeted small interfering RNA attenuated the inhibition of BDMC on 95D-cell migration and invasion. These findings provided direct evidence that BDMC inhibits 95D-cell migration and invasion. Furthermore, the inhibition of 95D-cell migration and invasion was associated with the downregulation of vimentin expression and the upregulation of E‑cadherin expression. Autophagy was involved in the anti‑cancer effects of BDMC on 95D cells. The present study provided novel insight into the underlying mechanisms of the anti-cancer effect of BDMC.
姜黄素是药用植物姜黄的一种活性成分，据报道其在多种癌症类型中具有抗转移活性并诱导自噬。双去甲氧基姜黄素（BDMC）是姜黄中存在的主要活性姜黄素衍生物之一，先前已证明其能在高转移性大细胞肺癌95D细胞中触发自噬。然而，BDMC诱导自噬对95D细胞侵袭和迁移的影响仍不清楚。因此，本研究调查了BDMC对高转移性大细胞肺癌95D细胞侵袭和迁移的影响。同时，我们观察了BDMC诱导的自噬对95D细胞迁移和侵袭的作用。Transwell分析表明，BDMC对95D细胞的迁移和侵袭具有抑制作用。此外，在用BDMC处理的95D细胞中，波形蛋白的表达下调，而E-钙黏蛋白的表达上调。此外，通过靶向Beclin1的小干扰RNA阻断自噬减弱了BDMC对95D细胞迁移和侵袭的抑制作用。这些发现提供了直接证据，证明BDMC抑制95D细胞的迁移和侵袭。此外，95D细胞迁移和侵袭的抑制与波形蛋白表达的下调和E-钙黏蛋白表达的上调有关。自噬参与了BDMC对95D细胞的抗癌作用。本研究为BDMC抗癌作用的潜在机制提供了新的见解。
