Qiao Wanqiong, Chiang Hao-Chun, Xie Hui, Levicky Rastislav
Dept. of Chemical & Biomolecular Engineering, NYU Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA.
Chem Commun (Camb). 2015 Dec 18;51(97):17245-8. doi: 10.1039/c5cc06674c.
Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behavior.
针对两种杂交探针(相同序列的DNA和不带电荷的吗啉代寡核苷酸),比较了在固体支持物上的杂交热力学与溶液中的杂交热力学。从离子强度、温度和表面行为方面讨论了杂交亲和力的趋势。