Giboda M, Pena E, Pividal J
Cesk Epidemiol Mikrobiol Imunol. 1989 Jan;38(1):18-26.
The importance of antigenic diversity in immunogenicity of population of P. falciparum was studied in regard of immunodiagnosis of tropical malaria. The sera from 42 adult non-immune persons with confirmed infection by P. falciparum and from 30 persons from Africa with confirmed infection by P. falciparum were examined with three antigens differing in geographic origin. Antigen A2 Gambia, M94 Thailand, FCQ2 Papua New Guinea were checked. The following results were obtained: 1) In the quantity of polyclonal antibodies detected higher frequency of higher titres and higher GMRT (statistically significant differences) were demonstrated with three antigens in the persons from the endemic areas of malaria. 2) Higher variation (antigenic diversity) in the quantity of polyclonal antibodies (difference up to 6 dilutions) in the non-immune population in contrast to semi-immune population where the differences were only at the level of two fold dilutions. 3) The correspondence of titres of polyclonal antibodies to all three geographically different antigens was statistically significantly (P less than 0.05) more frequent in the sera of semi-immune persons in contrast to non-immune individuals. 4) The results have shown that repeated infections in endemic areas of malaria with antigenically diverse strains induce the formation of polyclonal antibodies against all antigenic varieties and are better detectable by antigen of any antigenic variety.(ABSTRACT TRUNCATED AT 250 WORDS)
关于热带疟疾的免疫诊断，研究了恶性疟原虫群体免疫原性中抗原多样性的重要性。用三种地理来源不同的抗原检测了42名确诊感染恶性疟原虫的成年非免疫者和30名来自非洲确诊感染恶性疟原虫者的血清。检测了冈比亚的A2抗原、泰国的M94抗原、巴布亚新几内亚的FCQ2抗原。得到以下结果：1）在检测到的多克隆抗体数量方面，疟疾流行区人群中三种抗原的高滴度和高几何平均滴度（GMRT）出现频率更高（有统计学显著差异）。2）与半免疫人群相比，非免疫人群中多克隆抗体数量的变异（抗原多样性）更大（差异高达6倍稀释），而半免疫人群中差异仅在2倍稀释水平。3）与非免疫个体相比，半免疫者血清中针对所有三种地理上不同抗原的多克隆抗体滴度的对应性在统计学上更显著（P小于0.05）。4）结果表明，疟疾流行区反复感染抗原性不同的菌株会诱导形成针对所有抗原变体的多克隆抗体，并且用任何抗原变体的抗原都能更好地检测到。（摘要截短为250字）
