Wu Min, Ding Wen, Meng Junli, Ni Henmei, Li Ying, Ma Quanhong
School of Chemistry and Chemical Engineering, Southeast University.
Anal Sci. 2015;31(10):1027-33. doi: 10.2116/analsci.31.1027.
A novel biosensor was developed by immobilizing hemoglobin (Hb) on a glassy carbon electrode (GCE) modified with a composite of ZnO nano-rods and carbon nanofiber (CNF), a strong reducer, hydrazine, was firstly used to evaluate the electrochemical behavior of Hb on Hb/ZnO/CNF/GCE. UV-vis and circular dichroism (CD) spectra indicated the conformational structure of Hb interaction with ZnO/CNF was predominantly an α-helical structure. The modified electrodes were characterized by scanning electron microscopy (SEM), electron impedance spectroscopy (EIS), and cyclic voltammetry. Electrocatalytic mechanism of Hb to oxidation reaction of hydrazine was suggested. The bioelectrocatalytic activity, kinetic parameters of Michaelis-Menten constant (Km), stability and reproducibility were also investigated. A linear dependence of peak currents to the concentrations of hydrazine was observed in the range from 1.98 × 10(-5) to 1.71 × 10(-3) mol L(-1) with a correlation coefficient of 0.998, and a detection limit (S/N = 3) of 6.60 μmol L(-1) was estimated.
通过将血红蛋白（Hb）固定在由氧化锌纳米棒和碳纳米纤维（CNF）复合材料修饰的玻碳电极（GCE）上，开发了一种新型生物传感器。首先使用强还原剂肼来评估Hb在Hb/ZnO/CNF/GCE上的电化学行为。紫外可见光谱和圆二色光谱（CD）表明，Hb与ZnO/CNF相互作用的构象结构主要是α-螺旋结构。通过扫描电子显微镜（SEM）、电子阻抗谱（EIS）和循环伏安法对修饰电极进行了表征。提出了Hb对肼氧化反应的电催化机理。还研究了生物电催化活性、米氏常数（Km）的动力学参数、稳定性和重现性。在1.98×10⁻⁵至1.71×10⁻³mol L⁻¹范围内观察到峰电流与肼浓度呈线性关系，相关系数为0.998，估计检测限（S/N = 3）为6.60μmol L⁻¹。
