Li Hui, Yang Xue, Shi Wei, Ma Zhao, Feng Guang Kun, Yin Yan Ling, Fan Yan Xia, Jiang Jie
Department of Neurology, Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
Department of Neurology, Shandong Qilu Hospital, Jinan 250012, Shandong, China.
Biomed Environ Sci. 2015 Sep;28(9):691-5. doi: 10.3967/bes2015.098.
Recent studies showed that pathology of alcoholic encephalopathy was associated with cerebral vascular damage. TMP (tetramethyl- pyrazine) is widely used in the treatment of cerebrovascular diseases, however, it has not been reported whether TMP can relieve alcohol-induced cerebral vascular damages. The study was performed to investigate the learning and memory, cerebrovascular pathological changes and the expressions of vascular endothelial growth factor (VEGF) and serum levelsofendothelin-1 (ET-1) in the rat model of chronic alcoholic encephalopathy, and explore the effects of TMP intervention on alcoholic encephalopathy. In the present study, the rat model of chronic alcoholic encephalopathy was established by the gavage administration of alcohol; the learning and memory ability was tested by Morris water maze; the expression of VEGF was measured by RT-PCR and Western blot; and the serum levels of ET-1 was measured by radioimmunoassay. We found that alcohol intoxication impaired learning and memory, induced VEGF overexpression and increased ET 1 concentrations. TMP intervention improved learning abilities, increased the VEGF expression and reduced ET-1 level. These results indicate that TMP exhibits therapeutic effects on chronic alcoholic encephalopathy.
近期研究表明，酒精性脑病的病理变化与脑血管损伤有关。川芎嗪（TMP）广泛应用于脑血管疾病的治疗，然而，TMP能否减轻酒精诱导的脑血管损伤尚未见报道。本研究旨在探讨慢性酒精性脑病大鼠模型的学习记忆、脑血管病理变化以及血管内皮生长因子（VEGF）的表达和内皮素-1（ET-1）的血清水平，并探究TMP干预对酒精性脑病的影响。在本研究中，通过灌胃酒精建立慢性酒精性脑病大鼠模型；采用Morris水迷宫测试学习记忆能力；通过RT-PCR和蛋白质免疫印迹法检测VEGF的表达；采用放射免疫分析法检测血清ET-1水平。我们发现酒精中毒损害学习记忆，诱导VEGF过表达并增加ET-1浓度。TMP干预可改善学习能力，增加VEGF表达并降低ET-1水平。这些结果表明，TMP对慢性酒精性脑病具有治疗作用。
